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MSH6 Monoclonal antibody

MSH6 Monoclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

2E10B2

Cat no : 66172-1-Ig

Synonyms

G/T mismatch binding protein, GTBP, GTMBP, hMSH6, HNPCC5, HSAP, MSH6, MutS alpha 160 kDa subunit, mutS homolog 6 (E. coli), p160



Tested Applications

Positive WB detected inHEK-293 cells, HEK-293
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:200-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

IHCSee 3 publications below

Product Information

66172-1-Ig targets MSH6 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen MSH6 fusion protein Ag12645
Full Name mutS homolog 6 (E. coli)
Calculated molecular weight 153 kDa
Observed molecular weight 160 kDa
GenBank accession numberBC004246
Gene symbol MSH6
Gene ID (NCBI) 2956
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MSH6, also named as DNA mismatch repair protein Msh6 or GTMBP, is a 1360 amino acid protein, which contains one PWWP domain and belongs to the DNA mismatch repair MutS family. MSH6 localizes in the nucleus and is a component of the post-replicative DNA mismatch repair system. Msh2 and Msh6 form a protein complex required to repair mismatches generated during DNA replication.

Protocols

Product Specific Protocols
WB protocol for MSH6 antibody 66172-1-IgDownload protocol
IHC protocol for MSH6 antibody 66172-1-IgDownload protocol
IF protocol for MSH6 antibody 66172-1-IgDownload protocol
IP protocol for MSH6 antibody 66172-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Oncol Lett

HDAC10 expression is associated with DNA mismatch repair gene and is a predictor of good prognosis in colon carcinoma.

Authors - Xiangxiang Tao
humanIHC

J Surg Oncol

Clinicopathological and survival characteristic of mismatch repair status in ovarian clear cell carcinoma.

Authors - Jun Zhu
humanIHC

Surgery

Mismatch repair status as a beneficial predictor of fluorouracil-based adjuvant chemotherapy for pancreatic cancer.

Authors - Dingkong Liang