Tested Applications
| Positive WB detected in | mouse testis tissue |
| Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14251-1-AP targets MSRB3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5524 Product name: Recombinant human MSRB3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-185 aa of BC040053 Sequence: MSAFNLLHLVTKSQPVALRACGLPSGSCRDKKNCKVVFSQQELRKRLTPLQYHVTQEKGTESAFEGEYTHHKDPGIYKCVVCGTPLFKSETKFDSGSGWPSFHDVINSEAITFTDDFSYGMHRVETSCSQCGAHLGHIFDDGPRPTGKRYCINSAALSFTPADSSGTAEGGSGVASPAQADKAEL Predict reactive species |
| Full Name | methionine sulfoxide reductase B3 |
| Calculated Molecular Weight | 21 kDa |
| Observed Molecular Weight | 26 kDa |
| GenBank Accession Number | BC040053 |
| Gene Symbol | MSRB3 |
| Gene ID (NCBI) | 253827 |
| RRID | AB_2878035 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8IXL7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for MSRB3 antibody 14251-1-AP | Download protocol |
| WB protocol for MSRB3 antibody 14251-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MSRB3 Polyclonal antibody (Cat# 14251-1-AP) has 6 total citations. These publications appear in Free Radical Biology and Medicine, Journal of the Science of Food and Agriculture, Food Science and Biotechnology, Human Genetics, Journal of Food Biochemistry, and Journal of Medical Food. The research fields span oxidative stress and antioxidant responses (Nrf2 pathway), methionine metabolism, food science and nutrition, and congenital heart disease. All cited applications are Western blot in mouse or rat models.
| Species | Application | Title |
|---|---|---|
Free Radic Biol Med Loss of methionine sulfoxide reductases increases resistance to oxidative stress. | ||
J Sci Food Agric l-Methionine activates Nrf2-ARE pathway to induce endogenous antioxidant activity for depressing ROS-derived oxidative stress in growing rats. | ||
Food Sci Biotechnol Methionine strengthens anti-inflammation of rice protein via depressing NF-κB activation and stimulating Msr expression in rats fed cholesterol-enriched diets. | ||
Hum Genet Compound heterozygous mutation of the ASXL3 gene causes autosomal recessive congenital heart disease. | ||
J Food Biochem Rice protein stimulates endogenous antioxidant response attributed to methionine availability in growing rats. | ||
J Med Food Rice Protein Exerts Endogenous Antioxidant Capacity via Methionine Sulfoxide Reductase and the Nrf2 Antioxidant System Independent of Age. |
