MSRB3 Polyclonal antibody

MSRB3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 14251-1-AP

Synonyms

MSRB3

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Validation Data Gallery

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  WB analysis of mouse testis using 14251-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14251-1-AP (MSRB3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 14251-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14251-1-AP (MSRB3 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human testis using 14251-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14251-1-AP (MSRB3 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse testis tissue
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below

Product Information

14251-1-AP targets MSRB3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MSRB3 fusion protein Ag5524
• Full Name: methionine sulfoxide reductase B3
• Calculated Molecular Weight: 21 kDa
• Observed Molecular Weight: 26 kDa
• GenBank Accession Number: BC040053
• Gene Symbol: MSRB3
• Gene ID (NCBI): 253827
• RRID: AB_2878035
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Hum Genet; Compound heterozygous mutation of the ASXL3 gene causes autosomal recessive congenital heart disease.; Authors - Fang Fu
mouse	WB	Free Radic Biol Med; Loss of methionine sulfoxide reductases increases resistance to oxidative stress.; Authors - Lo Lai
rat	WB	J Med Food; Rice Protein Exerts Endogenous Antioxidant Capacity via Methionine Sulfoxide Reductase and the Nrf2 Antioxidant System Independent of Age.; Authors - Hui Li
rat	WB	Food Sci Biotechnol; Methionine strengthens anti-inflammation of rice protein via depressing NF-κB activation and stimulating Msr expression in rats fed cholesterol-enriched diets.; Authors - Zhengxuan Wang
rat	WB	J Food Biochem; Rice protein stimulates endogenous antioxidant response attributed to methionine availability in growing rats.; Authors - Zhengxuan Wang
rat	WB	J Sci Food Agric; l-Methionine activates Nrf2-ARE pathway to induce endogenous antioxidant activity for depressing ROS-derived oxidative stress in growing rats.; Authors - Zhengxuan Wang