MSX2 Monoclonal antibody

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  WB analysis using 68550-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68550-1-Ig (MSX2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68550-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68550-1-Ig (MSX2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 68550-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 68550-1-Ig (MSX2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 68550-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 68550-1-Ig (MSX2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MSX2 Monoclonal Antibody for WB, IHC, ELISA

Cat No. 68550-1-Ig

Clone No.1B1G3

Publications(1)

Host / Isotype

Mouse / IgG1

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Synonyms

1B1G3, Homeobox protein Hox-8, Homeobox protein MSX-2, HOX8

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Tested Applications

• Positive WB detected in: HT-1376 cells, SW 1990 cells, Saos-2 cells, HEK-293 cells, Caco-2 cells
• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

68550-1-Ig targets MSX2 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag33220

  Product name: Recombinant human MSX2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-267 aa of BC015509

  Sequence: MASPSKGNDLFSPDEEGPAVVAGPGPGPGGAEGAAEERRVKVSSLPFSVEALMSDKKPPKEASPLPAESASAGATLRPLLLSGHGAREAHSPGPLVKPFETASVKSENSEDGAAWMQEPGRYSPPPRHTSPTTCTLRKHKTNRKPRTPFTTSQLLALERKFRQKQYLSIAERAEFSSSLNLTETQVKIWFQNRRAKAKRLQEAELEKLKMAAKPMLPSSFSLPFPISSPLQAASIYGASYPFHRPVLPIPPVGLYATPVGYGMYHLS

• Full Name: msh homeobox 2
• Calculated Molecular Weight: 29 kDa
• Observed Molecular Weight: 28-35 kDa
• GenBank Accession Number: BC015509
• Gene Symbol: MSX2
• Gene ID (NCBI): 4488
• RRID: AB_3085254
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P35548
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MSX2 (Msh Homeobox 2) is a transcription factor belonging to the MSX homeobox gene family, which plays crucial roles in embryonic development, cell differentiation, and tissue homeostasis. The apparent molecular weight is larger than the calculated molecular weight of 29 kDa, which is likely due to posttranslational modification.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human,rat	WB,IHC	Biochem Pharmacol; TGF-β1/IL-11 positive loop between renal tubular epithelial cell and fibroblast promotes the ectopic calcification of renal interstitial fibroblasts in rats; Authors - Jinghua Zhong