MT1M Polyclonal antibody

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  IHC staining of human kidney using 17281-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17281-1-AP (MT1M Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 17281-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17281-1-AP (MT1M Antibody) at dilution of 1:50 (under 10x lens).

MT1M Polyclonal Antibody for IHC, ELISA

Cat No. 17281-1-AP

Publications(6)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Synonyms

metallothionein 1M, Metallothionein IM, MT 1M, MT IM, MT1, MT1K, MT1M

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Tested Applications

• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IF: See 2 publications below

Product Information

17281-1-AP targets MT1M in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag11048

  Product name: Recombinant human MT1M protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-61 aa of BC028280

  Sequence: MDPNCSCTTGVSCACTGSCKCKECKCTSCKKSCCSCCPVGCAKCAHGCVCKGTLENCSCCA

• Full Name: metallothionein 1M
• Calculated Molecular Weight: 61 aa, 6 kDa
• GenBank Accession Number: BC028280
• Gene Symbol: MT1M
• Gene ID (NCBI): 4499
• RRID: AB_2878376
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8N339
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human,mouse	WB	Autophagy; Therapeutic targeting of the USP2-E2F4 axis inhibits autophagic machinery essential for zinc homeostasis in cancer progression.; Authors - Wenjing Xiao
human	WB,IF	Environ Pollut; Biotransformation of graphene oxide within lung fluids could intensify its synergistic biotoxicity effect with cadmium by inhibiting cellular efflux of cadmium.; Authors - Jianqiang Zhu
human	IF	Clin Epigenetics; Epigenetic reactivation of tumor suppressor genes with CRISPRa technologies as precision therapy for hepatocellular carcinoma; Authors - Agustin Sgro
mouse	WB	ACS Nano; Amplification of Metalloregulatory Proteins in Macrophages by Bioactive ZnMn@SF Hydrogels for Spinal Cord Injury Repair; Authors - Xiaoliang Cui
human	WB	J Integr Med; Aloin blocks the malignant behavior of lung squamous cell carcinoma cells and M2 macrophage polarization by modulating the NR3C2/MT1M axis; Authors - Ying-Na Chen
human	WB	Int J Mol Sci; Specificity of the Metallothionein-1 Response by Cadmium-Exposed Normal Human Urothelial Cells.; Authors - Rhiannon V McNeill