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  • KD/KO Validated

MTHFD1 Polyclonal antibody

MTHFD1 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10794-1-AP

Synonyms

MTHFC, MTHFD, MTHFD1



Tested Applications

Positive WB detected inHEK-293 cells, human brain tissue, HuH-7 cells, K-562 cells, mouse kidney tissue
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman liver cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

10794-1-AP targets MTHFD1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MTHFD1 fusion protein Ag1225
Full Name methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase
Calculated molecular weight 101 kDa
Observed molecular weight 101 kDa
GenBank accession numberBC009806
Gene symbol MTHFD1
Gene ID (NCBI) 4522
RRIDAB_2147391
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MTHFD1 is a trifunctional enzyme involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism. MTHFD1 is associated with HCC and CRC progression. It has been reported that MTHFD-deficient patient fibroblasts exhibit enriched MTHFD1 in the nucleus which is critical for de novo dTMP synthesis.(PMID: 31133746, 30997850, 31421459, 25548164)

Protocols

Product Specific Protocols
WB protocol for MTHFD1 antibody 10794-1-APDownload protocol
IHC protocol for MTHFD1 antibody 10794-1-APDownload protocol
IP protocol for MTHFD1 antibody 10794-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

Oxidative stress inhibits distant metastasis by human melanoma cells.

Authors - Elena Piskounova
  • KD Validated
humanWB

J Pineal Res

Melatonin modulates metabolic remodeling in HNSCC by suppressing MTHFD1L-formate axis.

Authors - Li Cui
humanWB

Redox Biol

Cytosolic ME1 integrated with mitochondrial IDH2 supports tumor growth and metastasis.

Authors - Chang Shao
humanWB

Proc Natl Acad Sci U S A

Orthogonal genome-wide screens of bat cells identify MTHFD1 as a target of broad antiviral therapy.

Authors - Danielle E Anderson
  • KD Validated
mouseWB

Dev Cell

Illuminating NAD+ Metabolism in Live Cells and In Vivo Using a Genetically Encoded Fluorescent Sensor.

Authors - Yejun Zou
humanWB

Mol Oncol

Cysteine is a limiting factor for glioma proliferation and survival.

Authors - Victor Ruiz-Rodado

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Hua (Verified Customer) (08-26-2019)

Works well.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: liver, kidney, Hippocampus
MTHFD1 Antibody Western Blot, validation (1:500 dilution) in liver, kidney, Hippocampus (Cat no:10794-1-AP)