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  • KD/KO Validated

MTHFD1 Polyclonal antibody

MTHFD1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10794-1-AP

Synonyms

MTHFC, MTHFD, MTHFD1



Tested Applications

Positive WB detected inHEK-293 cells, human brain tissue, HuH-7 cells, K-562 cells, mouse kidney tissue
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman liver cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10794-1-AP targets MTHFD1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MTHFD1 fusion protein Ag1225
Full Name methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase
Calculated molecular weight 101 kDa
Observed molecular weight 101 kDa
GenBank accession numberBC009806
Gene symbol MTHFD1
Gene ID (NCBI) 4522
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MTHFD1 is a trifunctional enzyme involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism. MTHFD1 is associated with HCC and CRC progression. It has been reported that MTHFD-deficient patient fibroblasts exhibit enriched MTHFD1 in the nucleus which is critical for de novo dTMP synthesis.(PMID: 31133746, 30997850, 31421459, 25548164)

Protocols

Product Specific Protocols
WB protocol for MTHFD1 antibody 10794-1-APDownload protocol
IHC protocol for MTHFD1 antibody 10794-1-APDownload protocol
IP protocol for MTHFD1 antibody 10794-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Metab

Protein interaction and functional data indicate MTHFD2 involvement in RNA processing and translation.

Authors - Costas Koufaris
humanWB

Sci Rep

The Human Transient Receptor Potential Melastatin 2 Ion Channel Modulates ROS Through Nrf2.

Authors - Lei Bao
mouseWB

Dev Cell

Illuminating NAD+ Metabolism in Live Cells and In Vivo Using a Genetically Encoded Fluorescent Sensor.

Authors - Yejun Zou
humanWB

Redox Biol

Cytosolic ME1 integrated with mitochondrial IDH2 supports tumor growth and metastasis.

Authors - Chang Shao
humanWB

Nature

Oxidative stress inhibits distant metastasis by human melanoma cells.

Authors - Elena Piskounova
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Hua (Verified Customer) (08-26-2019)

Works well.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: liver, kidney, Hippocampus
MTHFD1 Antibody Western Blot, validation (1:500 dilution) in liver, kidney, Hippocampus (Cat no:10794-1-AP)