Validation Data Gallery
|Positive WB detected in
|HEK-293 cells, human brain tissue, HuH-7 cells, K-562 cells, mouse kidney tissue
|Positive IP detected in
|mouse kidney tissue
|Positive IHC detected in
|human liver cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)
|WB : 1:500-1:2000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:50-1:500
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
10794-1-AP targets MTHFD1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.
|Host / Isotype
|Rabbit / IgG
|MTHFD1 fusion protein Ag1225
|methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
MTHFD1 is a trifunctional enzyme involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism. MTHFD1 is associated with HCC and CRC progression. It has been reported that MTHFD-deficient patient fibroblasts exhibit enriched MTHFD1 in the nucleus which is critical for de novo dTMP synthesis.(PMID: 31133746, 30997850, 31421459, 25548164)
Oxidative stress inhibits distant metastasis by human melanoma cells.
J Pineal Res
Melatonin modulates metabolic remodeling in HNSCC by suppressing MTHFD1L-formate axis.
Cytosolic ME1 integrated with mitochondrial IDH2 supports tumor growth and metastasis.
Proc Natl Acad Sci U S A
Orthogonal genome-wide screens of bat cells identify MTHFD1 as a target of broad antiviral therapy.
Illuminating NAD+ Metabolism in Live Cells and In Vivo Using a Genetically Encoded Fluorescent Sensor.
Cysteine is a limiting factor for glioma proliferation and survival.
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Hua (Verified Customer) (08-26-2019)