mTOR Monoclonal antibody

mTOR Monoclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat and More (5)

Applications

WB, PLA, IP, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1G11A3

Cat no : 66888-1-Ig

Synonyms

FLJ44809, FRAP, FRAP1, FRAP2, Mammalian target of rapamycin, MTOR, RAFT1, Rapamycin target protein 1, RAPT1



Tested Applications

Positive WB detected inHeLa cells, ROS1728 cells, rat brain tissue, mouse brain tissue, HEK-293 cells, HepG2 cells, NCI-H1299 cells, Jurkat cells, THP-1 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue, human breast cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:5000-1:50000 for WB
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66888-1-Ig targets mTOR in WB, PLA, IP, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Cited Reactivity human, mouse, pig, rat, zebra finches
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen mTOR fusion protein Ag28395
Full Name FK506 binding protein 12-rapamycin associated protein 1
Calculated molecular weight 289 kDa
Observed molecular weight 250-289 kDa
GenBank accession numberNM_004958
Gene symbol FRAP1
Gene ID (NCBI) 2475
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MTOR, also named as FRAP1, FRAP, FRAP2 and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. MTOR is kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. MTOR has a calculated molecular mass of 289 kDa, and always can be detected at about 250 kDa due to some modifications (PMID: 14578359).

Protocols

Product Specific Protocols
WB protocol for mTOR antibody 66888-1-IgDownload protocol
IHC protocol for mTOR antibody 66888-1-IgDownload protocol
IF protocol for mTOR antibody 66888-1-IgDownload protocol
IP protocol for mTOR antibody 66888-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Med Rep

Curcumin inhibits high glucose‑induced inflammatory injury in human retinal pigment epithelial cells through the ROS‑PI3K/AKT/mTOR signaling pathway.

Authors - Zhenlong Ran
humanWB

Technol Cancer Res Treat

Knockdown of lncRNA OGFRP1 Inhibits Proliferation and Invasion of JEG-3 Cells Via AKT/mTOR Pathway.

Authors - Qian Meng
humanWB

J Exp Clin Cancer Res

Circ_0119872 promotes uveal melanoma development by regulating the miR-622/G3BP1 axis and downstream signalling pathways.

Authors - Shuting Liu
humanWB

J Exp Clin Cancer Res

Targeting SERT promotes tryptophan metabolism: mechanisms and implications in colon cancer treatment.

Authors - Di Ye
humanWB

Cell Death Dis

CircC16orf62 promotes hepatocellular carcinoma progression through the miR-138-5p/PTK2/AKT axis.

Authors - Shuai Zhang
pigWB

Res Vet Sci

Platycodon grandiflorus polysaccharides inhibit Pseudorabies virus replication via downregulating virus-induced autophagy.

Authors - Yuxiao Xing

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Rachel (Verified Customer) (05-24-2021)

Lovely antibody, very clean good siganl Cell lysates were run on JESS Simple Western

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/50
  • Cell Tissue Type: PC3 cells
mTOR Antibody Western Blot validation (1/50 dilution) in PC3 cells (Cat no:66888-1-Ig)