Validation Data Gallery
|Positive WB detected in||HeLa cells, ROS1728 cells, rat brain tissue, mouse brain tissue, HEK-293 cells, HepG2 cells, NCI-H1299 cells, Jurkat cells, THP-1 cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human colon cancer tissue, human breast cancer tissue, human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:5000-1:50000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:1000-1:4000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
66888-1-Ig targets mTOR in WB, PLA, IP, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
|Tested Reactivity||Human, Mouse, Rat|
|Cited Reactivity||human, mouse, pig, rat, zebra finches|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||mTOR fusion protein Ag28395|
|Full Name||FK506 binding protein 12-rapamycin associated protein 1|
|Calculated molecular weight||289 kDa|
|Observed molecular weight||250-289 kDa|
|GenBank accession number||NM_004958|
|Gene ID (NCBI)||2475|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
MTOR, also named as FRAP1, FRAP, FRAP2 and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. MTOR is kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. MTOR has a calculated molecular mass of 289 kDa, and always can be detected at about 250 kDa due to some modifications (PMID: 14578359).
|Product Specific Protocols|
|WB protocol for mTOR antibody 66888-1-Ig||Download protocol|
|IHC protocol for mTOR antibody 66888-1-Ig||Download protocol|
|IF protocol for mTOR antibody 66888-1-Ig||Download protocol|
|IP protocol for mTOR antibody 66888-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Rachel (Verified Customer) (05-24-2021)
Lovely antibody, very clean good siganl Cell lysates were run on JESS Simple Western