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mTOR Monoclonal antibody

mTOR Monoclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2a


Human, Mouse, Rat and More (8)







Cat no : 66888-1-Ig


FLJ44809, FRAP, FRAP1, FRAP2, Mammalian target of rapamycin, MTOR, RAFT1, Rapamycin target protein 1, RAPT1

Tested Applications

Positive WB detected inLNCaP cells, HEK-293 cells, HeLa cells, mouse brain tissue, rat brain tissue, ROS1728 cells, Caco-2 cells, HepG2 cells, Jurkat cells, NCI-H1299 cells, THP-1 cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue, human breast cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, HeLa cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66888-1-Ig targets mTOR in WB, IP, IF, PLA, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Cited Reactivityhuman, mouse, rat, goat, chicken, zebra finches, hamster, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen mTOR fusion protein Ag28395
Full Name FK506 binding protein 12-rapamycin associated protein 1
Calculated Molecular Weight 289 kDa
Observed Molecular Weight 250-289 kDa
GenBank Accession NumberNM_004958
Gene Symbol FRAP1
Gene ID (NCBI) 2475
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MTOR, also named as FRAP1, FRAP, FRAP2 and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. MTOR is kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. MTOR has a calculated molecular mass of 289 kDa, and always can be detected at about 250 kDa due to some modifications (PMID: 14578359).


Product Specific Protocols
WB protocol for mTOR antibody 66888-1-IgDownload protocol
IHC protocol for mTOR antibody 66888-1-IgDownload protocol
IF protocol for mTOR antibody 66888-1-IgDownload protocol
IP protocol for mTOR antibody 66888-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols




Nucleolar HEATR1 upregulated by mTORC1 signaling promotes hepatocellular carcinoma growth by dominating ribosome biogenesis and proteome homeostasis

Authors - Xiao-Mei Yang

Mol Cell

Lysosomal cyst(e)ine storage potentiates tolerance to oxidative stress in cancer cells

Authors - Lixin He

Mol Cancer

circEXOC6B interacting with RRAGB, an mTORC1 activator, inhibits the progression of colorectal cancer by antagonizing the HIF1A-RRAGB-mTORC1 positive feedback loop.

Authors - Xiaomin Li


Periplocin suppresses the growth of colorectal cancer cells by triggering LGALS3 (galectin 3)-mediated lysophagy

Authors - Kui Wang

Nat Commun

Location-specific inhibition of Akt reveals regulation of mTORC1 activity in the nucleus.

Authors - Xin Zhou

J Clin Invest

Up-regulated YB-1 protein promotes glioblastoma growth through an YB-1/CCT4/mLST8/mTOR pathway.

Authors - Jin-Zhu Wang


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Sun (Verified Customer) (01-05-2023)

strong band showed up around 200kDa region in HEK293T cells.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293T

Macarena (Verified Customer) (10-07-2022)

excellent band. Very concentrated.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: cell lines

Rachel (Verified Customer) (05-24-2021)

Lovely antibody, very clean good siganl Cell lysates were run on JESS Simple Western

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/50
  • Cell Tissue Type: PC3 cells
mTOR Antibody Western Blot validation (1/50 dilution) in PC3 cells (Cat no:66888-1-Ig)