mTOR Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

mTOR Monoclonal Antibody for WB, IP, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat

Applications

WB, IP, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G11A3

Cat no : 66888-1-PBS

Synonyms

FLJ44809, FRAP, FRAP1, FRAP2, Mammalian target of rapamycin, MTOR, RAFT1, Rapamycin target protein 1, RAPT1

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Validation Data Gallery

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  WB analysis using 66888-1-Ig (same clone as 66888-1-PBS)

  Various lysates (25 μg/lane) were subjected to SDS PAGE followed by western blot with 66888-1-Ig (MTOR antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66888-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66888-1-Ig (same clone as 66888-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66888-1-Ig (mTOR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66888-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66888-1-Ig (same clone as 66888-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66888-1-Ig (mTOR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66888-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66888-1-Ig (same clone as 66888-1-PBS)

  WB result of mTOR antibody (66888-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-mTOR transfected HeLa cells. This data was developed using the same antibody clone with 66888-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66888-1-Ig (same clone as 66888-1-PBS)

  Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 67778-1-Ig (Phospho-mTOR (Ser2448) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with RICTOR antibody (66867-2-Ig) and mTOR antibody (66888-1-Ig) subsequently. This data was developed using the same antibody clone with 66888-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66888-1-Ig (same clone as 66888-1-PBS)

  Non-treated and Rapamycin treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 67778-1-Ig (Phospho-mTOR (Ser2448) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with RICTOR antibody (66867-2-Ig) and mTOR antibody (66888-1-Ig) subsequently. This data was developed using the same antibody clone with 66888-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66888-1-Ig (same clone as 66888-1-PBS)

  Non-treated and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 67778-1-Ig (Phospho-mTOR (Ser2448) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with RICTOR antibody (66867-2-Ig) and mTOR antibody (66888-1-Ig) subsequently. This data was developed using the same antibody clone with 66888-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66888-1-Ig (same clone as 66888-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66888-1-Ig (mTOR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66888-1-PBS in a different storage buffer formulation.

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  WB analysis of ROS1728 using 66888-1-Ig (same clone as 66888-1-PBS)

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 66888-1-Ig (mTOR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66888-1-PBS in a different storage buffer formulation.

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  WB analysis of rat brain using 66888-1-Ig (same clone as 66888-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66888-1-Ig (mTOR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66888-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 66888-1-Ig (same clone as 66888-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66888-1-Ig (mTOR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66888-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 66888-1-Ig (same clone as 66888-1-PBS)

  IP result of anti-mTOR (IP:66888-1-Ig, 5ug; Detection:66888-1-Ig 1:20000) with HeLa cells lysate 1600 ug. This data was developed using the same antibody clone with 66888-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 66888-1-Ig (same clone as 66888-1-PBS)

  IP result of anti-mTOR (IP:66888-1-Ig, 5ug; Detection:66888-1-Ig 1:20000) with HeLa cells lysate 1600 ug. This data was developed using the same antibody clone with 66888-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66888-1-Ig (same clone as 66888-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66888-1-Ig (MTOR antibody) at dilution of 1:2500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66888-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66888-1-Ig (same clone as 66888-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66888-1-Ig (MTOR antibody) at dilution of 1:2500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66888-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66888-1-Ig (same clone as 66888-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66888-1-Ig (MTOR antibody) at dilution of 1:2500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66888-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66888-1-Ig (same clone as 66888-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66888-1-Ig (MTOR antibody) at dilution of 1:2500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66888-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66888-1-Ig (same clone as 66888-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66888-1-Ig (MTOR antibody) at dilution of 1:2500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66888-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66888-1-Ig (same clone as 66888-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66888-1-Ig (MTOR antibody) at dilution of 1:2500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66888-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 66888-1-Ig (same clone as 66888-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using mTOR antibody (66888-1-Ig, Clone: 1G11A3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66888-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66888-1-Ig (same clone as 66888-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using mTOR antibody (66888-1-Ig, Clone: 1G11A3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66888-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66888-1-Ig (same clone as 66888-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using mTOR antibody (66888-1-Ig, Clone: 1G11A3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66888-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66888-1-Ig (same clone as 66888-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using mTOR antibody (66888-1-Ig, Clone: 1G11A3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66888-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66888-1-PBS targets mTOR in WB, IP, IF, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: mTOR fusion protein Ag28395
• Full Name: FK506 binding protein 12-rapamycin associated protein 1
• Calculated Molecular Weight: 289 kDa
• Observed Molecular Weight: 250-289 kDa
• GenBank Accession Number: NM_004958
• Gene Symbol: FRAP1
• Gene ID (NCBI): 2475
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

MTOR, also named as FRAP1, FRAP, FRAP2 and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. MTOR is kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. MTOR has a calculated molecular mass of 289 kDa, and always can be detected at about 250 kDa due to some modifications (PMID: 14578359).