MTR Polyclonal antibody, PBS Only

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  WB analysis using 25896-1-AP (same clone as 25896-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 25896-1-AP (MTR antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25896-1-PBS in a different storage buffer formulation.

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  WB analysis of A375 using 25896-1-AP (same clone as 25896-1-PBS)

  A375 cells were subjected to SDS PAGE followed by western blot with 25896-1-AP (MTR Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25896-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse pancreas using 25896-1-AP (same clone as 25896-1-PBS)

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 25896-1-AP (MTR Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25896-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 25896-1-AP (same clone as 25896-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 25896-1-AP (MTR antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25896-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 25896-1-AP (same clone as 25896-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 25896-1-AP (MTR antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25896-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 25896-1-AP (same clone as 25896-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 25896-1-AP (MTR Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 25896-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 25896-1-AP (same clone as 25896-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 25896-1-AP (MTR Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 25896-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas using 25896-1-AP (same clone as 25896-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 25896-1-AP (MTR Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 25896-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas using 25896-1-AP (same clone as 25896-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 25896-1-AP (MTR Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 25896-1-PBS in a different storage buffer formulation.

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  IF Staining of A375 using 25896-1-AP (same clone as 25896-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed A375 cells using 25896-1-AP (MTR antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25896-1-PBS in a different storage buffer formulation.

MTR Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 25896-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Methionine synthase, 5-methyltetrahydrofolate--homocysteine methyltransferase, cblG, Cobalamin-dependent methionine synthase, EC:2.1.1.13

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Product Information

25896-1-PBS targets MTR in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MTR fusion protein Ag23156
• Full Name: 5-methyltetrahydrofolate-homocysteine methyltransferase
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: BC130616
• Gene Symbol: MTR
• Gene ID (NCBI): 4548
• RRID: AB_2880287
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q99707
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

MTR (Methionine synthase), one of two B12-dependent mammalian enzymes, catalyzes a methyl transfer from methyltetrahydrofolate to homocysteine, generating methionine and tetrahydrofolate (PMID: 9587027, 9013615). Methionine synthase levels in mammalian cells are influenced by folate status, and the ability of cells to grow in the absence of methionine is partly rate-limited by synthase activity levels. MTR loss depletes cells and tumors of reduced folate species (PMID: 34799699)