MTR Polyclonal antibody

MTR Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 25896-1-AP

Synonyms

cblG, Methionine synthase, MS, MTR



Tested Applications

Positive WB detected inA375 cells, mouse pancreas tissue
Positive IHC detected inhuman pancreas tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA375 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 25896-1-AP is MTR Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MTR fusion protein Ag23156
Full Name 5-methyltetrahydrofolate-homocysteine methyltransferase
Observed molecular weight 140 kDa
GenBank accession numberBC130616
Gene symbol MTR
Gene ID (NCBI) 4548
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MTR antibody 25896-1-APDownload protocol
IHC protocol for MTR antibody 25896-1-APDownload protocol
IF protocol for MTR antibody 25896-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Acta Pharmacol Sin

Exploring the protective effects of schizandrol A in acute myocardial ischemia mice by comprehensive metabolomics profiling integrated with molecular mechanism studies.

Authors - Qiong Lai
humanWB

Mol Cell

mTORC1 stimulates cell growth through SAM synthesis and m6A mRNA-dependent control of protein synthesis.

Authors - Elodie Villa
human

Cell Metab

Transsulfuration Activity Can Support Cell Growth upon Extracellular Cysteine Limitation.

Authors - Jiajun Zhu