MTUS1 Polyclonal antibody

• Featured Product
• KD/KO Validated

MTUS1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, CoIP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 13436-1-AP

Synonyms

AT2 receptor binding protein, ATBP, ATIP, GK1, KIAA1288, MP44, MTSG1, MTUS1

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Validation Data Gallery

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  WB analysis using 13436-1-AP

  Jurkat lysates were subjected to SDS PAGE followed by western blot with 13436-1-AP (MTUS1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 13436-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13436-1-AP (MTUS1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 13436-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13436-1-AP (MTUS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 13436-1-AP

  IP result of anti-MTUS1 (IP:13436-1-AP, 4ug; Detection:13436-1-AP 1:500) with mouse brain tissue lysate 6000ug.

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  IHC staining of human breast cancer using 13436-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13436-1-AP (MTUS1 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 13436-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13436-1-AP (MTUS1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human thyroid cancer using 13436-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 13436-1-AP (MTUS1 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 13436-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 13436-1-AP (MTUS1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of PC-3 using 13436-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-3 cells using 13436-1-AP (MTUS1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: Jurkat cells, K-562 cells, human brain tissue, mouse brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human breast cancer tissue, human thyroid cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:300-1:1200
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Product Information

13436-1-AP targets MTUS1 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MTUS1 fusion protein Ag4242
• Full Name: mitochondrial tumor suppressor 1
• Calculated Molecular Weight: 51 kDa, 141 kDa
• Observed Molecular Weight: 40-48 kDa, 80 kDa, 110-120 kDa, 270-280 kDa
• GenBank Accession Number: BC033842
• Gene Symbol: MTUS1
• Gene ID (NCBI): 57509
• RRID: AB_10638619
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MTUS1, also named as ATBP, ATIP, GK1, KIAA1288 and MTSG1, Belongs to the MTUS1 family. It cooperates with AGTR2 to inhibit ERK2 activation and cell proliferation. MTUS1 may be required for AGTR2 cell surface expression. Together with PTPN6, induces UBE2V2 expression upon angiotensin-II stimulation. Isoform 1 inhibits breast cancer cell proliferation, delays the progression of mitosis by prolonging metaphase and reduces tumor growth. MTUS1 up-regulation during cellular transition from proliferation to quiescence and differentiation. It is a potential tumor suppressor gene located at chromosome 8p21.3.22, near marker D8S254. According to the functional data and intracellular localization, MTUS1 also named as mitochondrial tumor suppressor gene 1 (MTSG1). One main feature common to all ATIP members is the presence of a large C-terminal coiled-coil domain that allows homo- and hetero-dimerization of these proteins. (PMID:12692079, 15123706). The antibody can recognize all the isoforms expect isoform 5(85-90 kDa). The antibody tested HomoDimer isoforms (80kd/110-120/280kd) in Jurkat cell.

Publications

Species	Application	Title
mouse	WB	Aging Cell; Activation of angiotensin-converting enzyme 2/angiotensin (1-7)/mas receptor axis triggers autophagy and suppresses microglia proinflammatory polarization via forkhead box class O1 signaling; Authors - Ruili Dang
human	WB,IF,CoIP	FASEB J; Microtubule associated tumor suppressor 1 interacts with mitofusins to regulate mitochondrial morphology in endothelial cells.; Authors - Yinfang Wang
human	WB,IF	J Mol Cell Cardiol; MTUS1 silencing promotes E-selectin production through p38 MAPK-dependent CREB ubiquitination in endothelial cells.; Authors - Yinfang Wang ; KD Validated
mouse	WB	Int J Med Sci; Light Emitting Diodes Irradiation Regulates miRNA-877-3p to Promote Cardiomyocyte Proliferation.; Authors - Xinlu Gao