MUC15 Recombinant monoclonal antibody

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  WB analysis of ACHN using 86987-1-RR

  ACHN cells were subjected to SDS PAGE followed by western blot with 86987-1-RR (MUC15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 86987-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86987-1-RR against Human MUC15 were performed. The affinity constant is 5.51 nM.

MUC15 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 86987-1-RR

Clone No.251970A2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

MUC 15, MUC-15, Mucin 15, Mucin-15, PAS3

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Tested Applications

• Positive WB detected in: ACHN cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86987-1-RR targets MUC15 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg5625

  Product name: recombinant human MUC15 protein

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 24-236 aa of NM_145650

  Sequence: KENQDINTTQNIAEVFKTMENKPISLESEANLNSDKENITTSNLKASHSPPLNLPNNSHGITDFSSNSSAEHSLGSLKPTSTISTSPPLIHSFVSKVPWNAPIADEDLLPISAHPNATPALSSENFTWSLVNDTVKTPDNSSITVSILSSEPTSPSVTPLIVEPSGWLTTNSDSFTGFTPYQEKTTLQPTLKFTNNSKLFPNTSDPQKENRNT

• Full Name: mucin 15, cell surface associated
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: NM_145650
• Gene Symbol: MUC15
• Gene ID (NCBI): 143662
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8N387-1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MUC15 is a transmembrane mucin that is ubiquitously expressed on the surface of epithelial cells as a membrane-bound glycoprotein. MUC15 plays a dual role in cellular processes, participating in cell adhesion, signal transduction, and regulating receptor tyrosine kinase activity. Its expression is frequently altered in various cancers, acting as a context-dependent tumor suppressor or promoter.(PMID: 32479243;16904320; 30420637)