MPDZ/MUPP1 Polyclonal antibody

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  WB analysis using 22583-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22583-1-AP (MUPP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

MPDZ/MUPP1 Polyclonal Antibody for WB, ELISA

Cat No. 22583-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

MPDZ, MUPP1

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, SK-N-SH cells, U-87 MG cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

22583-1-AP targets MPDZ/MUPP1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag18222

  Product name: Recombinant human MUPP1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 751-1100 aa of BC140793

  Sequence: LMFVNDVNLENSSLEEAVEALKGAPSGTVRIGVAKPLPLSPEEGYVSAKEDSFLYPPHSCEEAGLADKPLFRADLALVGTNDADLVDESTFESPYSPENDSIYSTQASILSLHGSSCGDGLNYGSSLPSSPPKDVIENSCDPVLDLHMSLEELYTQNLLQRQDENTPSVDISMGPASGFTINDYTPANAIEQQYECENTIVWTESHLPSEVISSAELPSVLPDSAGKGSEYLLEQSSLACNAECVMLQNVSKESFERTINIAKGNSSLGMTVSANKDGLGMIVRSIIHGGAISRDGRIAIGDCILSINEESTISVTNAQARAMLRRHSLIGPDIKITYVPAEHLEEFKIS

• Full Name: multiple PDZ domain protein
• Calculated Molecular Weight: 2070 aa, 222 kDa
• Observed Molecular Weight: 250 kDa
• GenBank Accession Number: BC140793
• Gene Symbol: MPDZ
• Gene ID (NCBI): 8777
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O75970
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The PDZ domain is a protein motif that mediates protein-protein interactions. MUPP1(multi-PDZ domain protein-1) has 13 PDZ domains, each containing the structural characteristics of a prototypic PDZ motif, but lacks a catalytic domain. MUPP1 belongs to the PDZ domain protein family and is a structural homologant of PATJ (PALS1-associated tight-linking protein) (PMID: 23880463). MUPP1 is a scaffold protein and plays an important role in cytoskeletal organization, cell polarity, and cell proliferation(PMID: 25662616). Mutations in MUPP1 are associated with congenital hydrocephalus(PMID: 23240096).