MUS81 Recombinant monoclonal antibody

• 

  WB analysis using 87462-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87462-1-RR (MUS81 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  Affinity of 87462-1-RR

  Biolayer interferometry (BLl) kinetic assays of 87462-1-RR against Human MUS81 were performed. The affinity constant is below 1 pM.

MUS81 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 87462-1-RR

Clone No.252834C7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

EC:3.1.22.-

---

Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, L02 cells, SH-SY5Y cells, NIH/3T3 cells, HSC-T6 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87462-1-RR targets MUS81 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag1476

  Product name: Recombinant human MUS81 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 202-551 aa of BC009999

  Sequence: RYSLTPEGLELAQKLAESEGLSLLNVGIGPKEPPGEETAVPGAASAELASEAGVQQQPLELRPGEYRVLLCVDIGETRGGGHRPELLRELQRLHVTHTVRKLHVGDFVWVAQETNPRDPANPGELVLDHIVERKRLDDLCSSIIDGRFREQKFRLKRCGLERRVYLVEEHGSVHNLSLPESTLLQAVTNTQVIDGFFVKRTADIKESAAYLALLTRGLQRLYQGHTLRSRPWGTPGNPESGAMTSPNPLCSLLTFSDFNAGAIKNKAQSVREVFARQLMQVRGVSGEKAAALVDRYSTPASLLAAYDACATPKEQETLLSTIKCGRLQRNLGPALSRTLSQLYCSYGPLT

• Full Name: MUS81 endonuclease homolog (S. cerevisiae)
• Calculated Molecular Weight: 61 kDa
• Observed Molecular Weight: 52-72 kDa
• GenBank Accession Number: BC009999
• Gene Symbol: MUS81
• Gene ID (NCBI): 80198
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q96NY9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The Crossover junction endonuclease MUS81 has associated endonuclease activity against structure-specific oligonucleotide substrates, including synthetic Holliday junctions(PMID:11741546). It interacts with EME1 and EME2 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. The open reading frame of human MUS81 predicts a translation product of 551 amino acids with a molecular mass of 59 kD. There are some reports showed that the enzyme can express a 72 kDa protein in human tissues(PMID:12724407).