MXRA5 Polyclonal antibody

• Featured Product
• KD/KO Validated

MXRA5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 25472-1-AP

Synonyms

Adlican, MXRA5

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Validation Data Gallery

• 

  WB analysis using 25472-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25472-1-AP (MXRA5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 25472-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25472-1-AP (MXRA5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 25472-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25472-1-AP (MXRA5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 25472-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 25472-1-AP (MXRA5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 25472-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 25472-1-AP (MXRA5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 25472-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25472-1-AP (MXRA5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 25472-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25472-1-AP (MXRA5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 25472-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25472-1-AP (MXRA5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 25472-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25472-1-AP (MXRA5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells
• Positive IHC detected in: human colon cancer tissue, human kidney tissue, human liver tissue, mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 2 publications below

Product Information

25472-1-AP targets MXRA5 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: matrix-remodelling associated 5
• Calculated Molecular Weight: 312 kDa
• Observed Molecular Weight: 163 kDa, 312 kDa
• GenBank Accession Number: NM_015419
• Gene Symbol: MXRA5
• Gene ID (NCBI): 25878
• RRID: AB_2918088
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MXRA5, or adlican, is an adhesion proteoglycan involved in extracellular matrix (ECM) remodeling and cell-cell adhesion (PMID: 22696596). In kidney, MXRA5 has anti-inflammatory and anti-fibrotic properties by limiting the induction of chemokines, fibronectin and collagen expression in response to TGB1 and pro-inflammatory stimuli (PMID: 27599751). MXRA5 was identified as a novel biomarker in colorectal cancer, mainly located in the cytoplasm of CRC cells (PMID:23420087). Exome sequencing identified MXRA5 as a novel cancer gene mutated in non-small cell lung carcinoma (PMID: 22696596). MXRA5 was detected 163 kDa in human proximal tubular cells (PMID:27599751).

Publications

Species	Application	Title
human	IHC,WB	Sci Rep; Public RNA-seq data-based identification and functional analyses reveal that MXRA5 retains proliferative and migratory abilities of dental pulp stem cells; Authors - Kazuma Yoshida ; KD Validated
human	IHC	Am J Cancer Res; Single-cell analysis reveals one cancer-associated fibroblasts subtype linked to metastasis in breast cancer: MXRA5 as a potential novel marker for prognosis; Authors - Huifang Deng