Tested Applications
| Positive WB detected in | HEK-293 cells |
| Positive IHC detected in | human colon cancer tissue, human kidney tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25472-1-AP targets MXRA5 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | matrix-remodelling associated 5 |
| Calculated Molecular Weight | 312 kDa |
| Observed Molecular Weight | 163 kDa, 312 kDa |
| GenBank Accession Number | NM_015419 |
| Gene Symbol | MXRA5 |
| Gene ID (NCBI) | 25878 |
| RRID | AB_2918088 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NR99 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MXRA5, or adlican, is an adhesion proteoglycan involved in extracellular matrix (ECM) remodeling and cell-cell adhesion (PMID: 22696596). In kidney, MXRA5 has anti-inflammatory and anti-fibrotic properties by limiting the induction of chemokines, fibronectin and collagen expression in response to TGB1 and pro-inflammatory stimuli (PMID: 27599751). MXRA5 was identified as a novel biomarker in colorectal cancer, mainly located in the cytoplasm of CRC cells (PMID:23420087). Exome sequencing identified MXRA5 as a novel cancer gene mutated in non-small cell lung carcinoma (PMID: 22696596). MXRA5 was detected 163 kDa in human proximal tubular cells (PMID:27599751).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for MXRA5 antibody 25472-1-AP | Download protocol |
| WB protocol for MXRA5 antibody 25472-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MXRA5 antibody (Cat# 25472-1-AP) has 3 total citations. These appear in Scientific Reports, Journal of Cancer, and American Journal of Cancer Research. The research fields span dental pulp stem cell biology (proliferation and migration), glioblastoma immune signature and prognosis, and breast cancer metastasis via cancer-associated fibroblasts. All studies used human samples with WB and/or IHC applications.
| Species | Application | Title |
|---|---|---|
Sci Rep Public RNA-seq data-based identification and functional analyses reveal that MXRA5 retains proliferative and migratory abilities of dental pulp stem cells
| ||
J Cancer Identification of an Immune signature assisted prognosis, and immunotherapy prediction for IDH wildtype glioblastoma | ||
Am J Cancer Res Single-cell analysis reveals one cancer-associated fibroblasts subtype linked to metastasis in breast cancer: MXRA5 as a potential novel marker for prognosis |
