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c-MYC Polyclonal antibody
c-MYC Polyclonal Antibody for FC, IF, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (6)
Applications
WB, IP, IF, FC, EMSA, CoIP, chIP, ELISA
Conjugate
Unconjugated
396
Cat no : 10828-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, HL-60 cells, human placenta tissue, HepG2 cells, A549 cells, HT-29 cells, Jurkat cells and Raji cells., MCF-7 cells, SH-SY5Y cells |
Positive IP detected in | MCF-7 cells |
Positive IF detected in | U2OS cells, HEK-293 cells |
Positive FC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:12000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 16 publications below |
WB | See 352 publications below |
IHC | See 38 publications below |
IF | See 23 publications below |
IP | See 10 publications below |
CoIP | See 5 publications below |
ChIP | See 7 publications below |
Product Information
10828-1-AP targets c-MYC in WB, IP, IF, FC, EMSA, CoIP, chIP, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse, rat, chicken, mosue, P. lilacinum, pig, sheep |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | c-MYC fusion protein Ag1263 |
Full Name | v-myc myelocytomatosis viral oncogene homolog (avian) |
Calculated molecular weight | 49 kDa |
Observed molecular weight | 62-65 kDa, 50 kDa |
GenBank accession number | BC000141 |
Gene symbol | MYC |
Gene ID (NCBI) | 4609 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Function
c-Myc (also known as Myc), together with l-Myc and n-Myc, belongs to the Myc family of transcription factors. c-Myc has a basic helix-loop-helix leucine zipper domain and through heterodimerization can bind and regulate the transcriptional activity of genes, either by repression or activation. It is a key player in the regulation of cell growth and cell cycle progression and acts as a proto-oncogene.
Tissue specificity
c-Myc is ubiquitously expressed in almost all cell types and its expression positively correlates with tissue proliferative capacity. c-Myc is also expressed during embryogenesis and is upregulated in many cancer types.
Involvement in disease
· Upregulated in many cancer types, especially in aggressive, poorly differentiated tumors.
· Mutations in the MYC gene and breakpoint translocations within the MYC gene cause Burkitt lymphoma.
Isoforms
There are 3 different isoforms of c-Myc: c-Myc1, c-Myc2, and c-MycS (PMID: 16260605). They differ in molecular size, can be preferentially expressed during cell growth, and are reported to be functionally distinct.
Post-translational modifications
c-Myc is subject to various post-translational modifications, including phosphorylation, acetylation, and ubiquitinylation (PMID: 16987807), which regulate its activity.
Cellular localization
c-Myc localizes to the nucleus but can also be present in the cytoplasm of certain cancer types.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for c-MYC antibody 10828-1-AP | Download protocol |
IF protocol for c-MYC antibody 10828-1-AP | Download protocol |
IP protocol for c-MYC antibody 10828-1-AP | Download protocol |
FC protocol for c-MYC antibody 10828-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Stem Cell Res Ther Exosomes from glioma cells induce a tumor-like phenotype in mesenchymal stem cells by activating glycolysis. | ||
J Exp Clin Cancer Res LPCAT1 promotes brain metastasis of lung adenocarcinoma by up-regulating PI3K/AKT/MYC pathway. | ||
Cancer Med A novel tumor suppressor SPINK5 targets Wnt/β-catenin signaling pathway in esophageal cancer. | ||
ACS Appl Mater Interfaces ROS-Responsive Polymeric Micelles for Triggered Simultaneous Delivery of PLK1 Inhibitor/miR-34a and Effective Synergistic Therapy in Pancreatic Cancer. | ||
Oncogene AKIP1 promotes early recurrence of hepatocellular carcinoma through activating the Wnt/β-catenin/CBP signaling pathway. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Anna-Maria (Verified Customer) (08-13-2019) | This antibody worked as expected for western blotting
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FH Christine (Verified Customer) (11-13-2018) | The antibody worked better than the Abcam c-Myc antibody. However, I did not get clear nuclear staining but rather a mix of nuclear staining with cytoplasmic staining. This could be due non-optimized conditions.
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FH Claudio (Verified Customer) (12-14-2017) | We tested the antibody in IHC/IFL on mouse brain tumors that showed c-myc upregulation at the mRNA level.
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