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c-MYC Antibody 106 Publications

Rabbit Polyclonal| Catalog number: 10828-1-AP

Featured Product KD/KO validated

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2 customer reviews

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Con: 43 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, FC, CoIP, ELISA
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Images See all

c-MYC Antibody WB HepG2 cells 10828-1-AP

Various lysates were subjected to SDS PAGE followed by western blot with 10828-1-AP (c-MYC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

c-MYC Antibody IHC human gliomas tissue 10828-1-AP

Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10828-1-AP (c-MYC antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

c-MYC Antibody FC HeLa cells 10828-1-AP

1X10^6 HeLa cells were stained with 0.20ug c-MYC antibody (10828-1-AP, red) and control antibody (blue). Fixed with 90% MeOH

c-MYC Antibody IP HL-60 cells 10828-1-AP

IP Result of anti-c-MYC (IP:10828-1-AP, 3ug; Detection:10828-1-AP 1:500) with HL-60 cells lysate 1200ug.

c-MYC Antibody IF NCCIT cell 10828-1-AP

Immunofluorescent analysis of ( 4% PFA ) fixed NCCIT cell using 10828-1-AP(c-MYC antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse, rat
Positive WB detected in:
HepG2 cells, HL-60 cells, human placenta tissue
Positive IP detected in:
HL-60 cells
Positive IHC detected in:
human gliomas tissue, human lymphoma tissue, mouse embryo tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
NCCIT cell
Positive FC detected in:
HeLa cells
Recommended dilution:
WB : 1:1000-1:4000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:50-1:500
IF : 1:50-1:500
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
c-MYC fusion protein Ag1263
Full name:
v-myc myelocytomatosis viral oncogene homolog (avian)
Calculated molecular weight:
49 kDa
Observed molecular weight:
62-65 kDa, 50 kDa
GenBank accession number:
BC000141
Gene ID (NCBI):
4609
Gene symbol
MYC
Synonyms
bHLHe39, c Myc, c-Myc, MRTL, MYC, Myc proto oncogene protein, Proto oncogene c Myc, Transcription factor p64

Background

Function

c-Myc (also known as Myc), together with l-Myc and n-Myc, belongs to the Myc family of transcription factors. c-Myc has a basic helix-loop-helix leucine zipper domain and through heterodimerization can bind and regulate the transcriptional activity of genes, either by repression or activation. It is a key player in the regulation of cell growth and cell cycle progression and acts as a proto-oncogene.

Tissue specificity

c-Myc is ubiquitously expressed in almost all cell types and its expression positively correlates with tissue proliferative capacity. c-Myc is also expressed during embryogenesis and is upregulated in many cancer types.

Involvement in disease

·         Upregulated in many cancer types, especially in aggressive, poorly differentiated tumors.

·         Mutations in the MYC gene and breakpoint translocations within the MYC gene cause Burkitt lymphoma.

Isoforms

There are 3 different isoforms of c-Myc: c-Myc1, c-Myc2, and c-MycS (PMID: 16260605). They differ in molecular size, can be preferentially expressed during cell growth, and are reported to be functionally distinct.

Post-translational modifications

c-Myc is subject to various post-translational modifications, including phosphorylation, acetylation, and ubiquitinylation (PMID: 16987807), which regulate its activity.

Cellular localization

c-Myc localizes to the nucleus but can also be present in the cytoplasm of certain cancer types.


Customer Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

Share your scientific insight on this antibody

c-MYC Rabbit Polyclonal antibody

Christine (Verified Customer), 11-13-2018
Applications
Immunohistochemistry,
Primary Antibody Dilution
1 to 300
No image submitted with review

No image submitted with review

Cell/Tissue Type
Colon
Lot Number
The antibody worked better than the Abcam c-Myc antibody. However, I did not get clear nuclear staining but rather a mix of nuclear staining with cytoplasmic staining. This could be due non-optimized conditions.

c-MYC Rabbit Polyclonal antibody

Claudio (Verified Customer), 12-14-2017
Applications
Immunohistochemistry, Immunofluorescence
Primary Antibody Dilution
100
Secondary Antibody Dilution
600
No image submitted with review

No image submitted with review

Cell/Tissue Type
Mouse brain tumors with c-myc upregulation (determined at the mRNA level)
Lot Number
00017880
We tested the antibody in IHC/IFL on mouse brain tumors that showed c-myc upregulation at the mRNA level.
Proteintech Response
Thank you for your review. We will add this to our siRNA pipeline and re-QC it.

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