Back

c-MYC Antibody 78 Publications

Rabbit Polyclonal| Catalog number: 10828-1-AP

Featured Product KD/KO validated

  • Print page
  • Download PDF

Be the first to review this product

-_-

Freight/Packing

Con: 43 μg/150 μl

Choose size:

Please visit your regions distributor:

Request quote for
bulk/custom formulations

Related Primary Antibodies
Special offer: Add control/tag antibody for $99
Load more

Tested Applications

Applications:
WB, IP, IHC, IF, FC, CoIP, ELISA
Application not tested? Please see our Guarantee


Images See all

c-MYC Antibody IHC human gliomas tissue 10828-1-AP

Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 10828-1-AP (c-MYC antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

c-MYC Antibody FC HeLa cells 10828-1-AP

1X10^6 HeLa cells were stained with 0.20ug c-MYC antibody (10828-1-AP, red) and control antibody (blue). Fixed with 90% MeOH

c-MYC Antibody WB Raji cells 10828-1-AP

Raji cells were subjected to SDS PAGE followed by western blot with 10828-1-AP(c-MYC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

c-MYC Antibody IP HL-60 cells 10828-1-AP

IP Result of anti-c-MYC (IP:10828-1-AP, 3ug; Detection:10828-1-AP 1:500) with HL-60 cells lysate 1200ug.

c-MYC Antibody IF NCCIT cell 10828-1-AP

Immunofluorescent analysis of ( 4% PFA ) fixed NCCIT cell using 10828-1-AP(c-MYC antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse, rat
Positive WB detected in:
Raji cells, HL-60 cells, human placenta tissue
Positive IP detected in:
HL-60 cells
Positive IHC detected in:
human gliomas tissue, human lymphoma tissue, mouse embryo tissue
Positive IF detected in:
NCCIT cell
Positive FC detected in:
HeLa cells
Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:50-1:500
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
c-MYC fusion protein Ag1263
Full name:
v-myc myelocytomatosis viral oncogene homolog (avian)
Calculated molecular weight:
49 kDa
Observed molecular weight:
62-65 kDa, 50 kDa
GenBank accession number:
BC000141
Gene ID (NCBI):
4609
Gene symbol
MYC
Synonyms
bHLHe39, c Myc, c-Myc, MRTL, MYC, Myc proto oncogene protein, Proto oncogene c Myc, Transcription factor p64

Background

MYC contains one basic helix-loop-helix (bHLH) domain. This protein is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. It seems to activate the transcription of growth-related genes. It binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. This antibody is a rabbit polyclonal antibody raised against recombinant protein of human MYC. The 50kDa band recognized by antibody is the native form of MYC, while the other bands, between 60-70kDa, are the phosphorylated form of MYC [PMID: 12189186].


Back
to top