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- KD/KO Validated
c-MYC Polyclonal antibody
c-MYC Polyclonal Antibody for FC, IF, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (3)
Applications
WB, IP, IF, FC, EMSA, CoIP, chIP,ELISA
Conjugate
Unconjugated
277
Cat no : 10828-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, HL-60 cells, human placenta tissue |
| Positive IP detected in | HL-60 cells |
| Positive IF detected in | NCCIT cell |
| Positive FC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 9 publications below |
| WB | See 244 publications below |
| IHC | See 23 publications below |
| IF | See 14 publications below |
| IP | See 2 publications below |
| CoIP | See 3 publications below |
| ChIP | See 3 publications below |
Product Information
10828-1-AP targets c-MYC in WB, IP, IF, FC, EMSA, CoIP, chIP,ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mosue, mouse, pig, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | c-MYC fusion protein Ag1263 |
| Full Name | v-myc myelocytomatosis viral oncogene homolog (avian) |
| Calculated molecular weight | 49 kDa |
| Observed molecular weight | 62-65 kDa, 50 kDa |
| GenBank accession number | BC000141 |
| Gene symbol | MYC |
| Gene ID (NCBI) | 4609 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Function
c-Myc (also known as Myc), together with l-Myc and n-Myc, belongs to the Myc family of transcription factors. c-Myc has a basic helix-loop-helix leucine zipper domain and through heterodimerization can bind and regulate the transcriptional activity of genes, either by repression or activation. It is a key player in the regulation of cell growth and cell cycle progression and acts as a proto-oncogene.
Tissue specificity
c-Myc is ubiquitously expressed in almost all cell types and its expression positively correlates with tissue proliferative capacity. c-Myc is also expressed during embryogenesis and is upregulated in many cancer types.
Involvement in disease
· Upregulated in many cancer types, especially in aggressive, poorly differentiated tumors.
· Mutations in the MYC gene and breakpoint translocations within the MYC gene cause Burkitt lymphoma.
Isoforms
There are 3 different isoforms of c-Myc: c-Myc1, c-Myc2, and c-MycS (PMID: 16260605). They differ in molecular size, can be preferentially expressed during cell growth, and are reported to be functionally distinct.
Post-translational modifications
c-Myc is subject to various post-translational modifications, including phosphorylation, acetylation, and ubiquitinylation (PMID: 16987807), which regulate its activity.
Cellular localization
c-Myc localizes to the nucleus but can also be present in the cytoplasm of certain cancer types.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for c-MYC antibody 10828-1-AP | Download protocol |
| IF protocol for c-MYC antibody 10828-1-AP | Download protocol |
| IP protocol for c-MYC antibody 10828-1-AP | Download protocol |
| FC protocol for c-MYC antibody 10828-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Stem Cell Res Ther Exosomes from glioma cells induce a tumor-like phenotype in mesenchymal stem cells by activating glycolysis. | ||
J Exp Clin Cancer Res LPCAT1 promotes brain metastasis of lung adenocarcinoma by up-regulating PI3K/AKT/MYC pathway. | ||
Cancer Med A novel tumor suppressor SPINK5 targets Wnt/β-catenin signaling pathway in esophageal cancer. | ||
ACS Appl Mater Interfaces ROS-Responsive Polymeric Micelles for Triggered Simultaneous Delivery of PLK1 Inhibitor/miR-34a and Effective Synergistic Therapy in Pancreatic Cancer. | ||
Oncogene AKIP1 promotes early recurrence of hepatocellular carcinoma through activating the Wnt/β-catenin/CBP signaling pathway. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Anna-Maria (Verified Customer) (08-13-2019) | This antibody worked as expected for western blotting
|
FH Christine (Verified Customer) (11-13-2018) | The antibody worked better than the Abcam c-Myc antibody. However, I did not get clear nuclear staining but rather a mix of nuclear staining with cytoplasmic staining. This could be due non-optimized conditions.
|
FH Claudio (Verified Customer) (12-14-2017) | We tested the antibody in IHC/IFL on mouse brain tumors that showed c-myc upregulation at the mRNA level.
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