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- KD/KO Validated
CoraLite®488-conjugated c-MYC Polyclonal antibody
c-MYC Polyclonal Antibody for FC (Intra), IF
Host / Isotype
Rabbit / IgG
IF, FC (Intra)
CoraLite®488 Fluorescent Dye
Cat no : CL488-10828
|Positive IF detected in||HEK-293 cells|
|Positive FC detected in||HeLa cells|
|Immunofluorescence (IF)||IF : 1:300-1:1200|
|Sample-dependent, check data in validation data gallery|
CL488-10828 targets c-MYC in IF, FC (Intra) applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||c-MYC fusion protein Ag1263|
|Full Name||v-myc myelocytomatosis viral oncogene homolog (avian)|
|Calculated molecular weight||49 kDa|
|Observed molecular weight||62-65 kDa, 50 kDa|
|GenBank accession number||BC000141|
|Gene ID (NCBI)||4609|
|Conjugate||CoraLite®488 Fluorescent Dye|
|Excitation/Emission maxima wavelengths||488 nm / 515 nm|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.|
|Storage Conditions||Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.|
c-Myc (also known as Myc), together with l-Myc and n-Myc, belongs to the Myc family of transcription factors. c-Myc has a basic helix-loop-helix leucine zipper domain and through heterodimerization can bind and regulate the transcriptional activity of genes, either by repression or activation. It is a key player in the regulation of cell growth and cell cycle progression and acts as a proto-oncogene.
c-Myc is ubiquitously expressed in almost all cell types and its expression positively correlates with tissue proliferative capacity. c-Myc is also expressed during embryogenesis and is upregulated in many cancer types.
Involvement in disease
· Upregulated in many cancer types, especially in aggressive, poorly differentiated tumors.
· Mutations in the MYC gene and breakpoint translocations within the MYC gene cause Burkitt lymphoma.
There are 3 different isoforms of c-Myc: c-Myc1, c-Myc2, and c-MycS (PMID: 16260605). They differ in molecular size, can be preferentially expressed during cell growth, and are reported to be functionally distinct.
c-Myc is subject to various post-translational modifications, including phosphorylation, acetylation, and ubiquitinylation (PMID: 16987807), which regulate its activity.
c-Myc localizes to the nucleus but can also be present in the cytoplasm of certain cancer types.