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CoraLite®555-conjugated c-MYC Polyclonal antibody

c-MYC Polyclonal Antibody for FC (Intra), IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF, FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

Cat no : CL555-10828

Synonyms

bHLHe39, c Myc, c-MYC, MRTL, MYC, Myc proto oncogene protein, Proto oncogene c Myc, Transcription factor p64



Tested Applications

Positive IF detected inHEK-293 cells
Positive FC detected inRaji cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL555-10828 targets c-MYC in IF, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen c-MYC fusion protein Ag1263
Full Name v-myc myelocytomatosis viral oncogene homolog (avian)
Calculated molecular weight 49 kDa
Observed molecular weight 62-65 kDa, 50 kDa
GenBank accession numberBC000141
Gene symbol MYC
Gene ID (NCBI) 4609
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission maxima wavelengths555 nm / 570 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

Function

c-Myc (also known as Myc), together with l-Myc and n-Myc, belongs to the Myc family of transcription factors. c-Myc has a basic helix-loop-helix leucine zipper domain and through heterodimerization can bind and regulate the transcriptional activity of genes, either by repression or activation. It is a key player in the regulation of cell growth and cell cycle progression and acts as a proto-oncogene.

Tissue specificity

c-Myc is ubiquitously expressed in almost all cell types and its expression positively correlates with tissue proliferative capacity. c-Myc is also expressed during embryogenesis and is upregulated in many cancer types.

Involvement in disease

·         Upregulated in many cancer types, especially in aggressive, poorly differentiated tumors.

·         Mutations in the MYC gene and breakpoint translocations within the MYC gene cause Burkitt lymphoma.

Isoforms

There are 3 different isoforms of c-Myc: c-Myc1, c-Myc2, and c-MycS (PMID: 16260605). They differ in molecular size, can be preferentially expressed during cell growth, and are reported to be functionally distinct.

Post-translational modifications

c-Myc is subject to various post-translational modifications, including phosphorylation, acetylation, and ubiquitinylation (PMID: 16987807), which regulate its activity.

Cellular localization

c-Myc localizes to the nucleus but can also be present in the cytoplasm of certain cancer types.


Protocols

Product Specific Protocols
IF protocol for CL555 c-MYC antibody CL555-10828Download protocol
FC protocol for CL555 c-MYC antibody CL555-10828Download protocol
Standard Protocols
Click here to view our Standard Protocols