MYC-tag Monoclonal antibody

MYC-tag Monoclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Recombinant protein and More (2)

Applications

WB, IP, IF, CoIP, ELISA

Conjugate

Unconjugated

CloneNo.

2D11A8

Cat no : 66004-1-Ig

Synonyms


"MYC-tag Antibodies" Comparison

View side-by-side comparison of MYC-tag antibodies from other vendors to find the one that best suits your research needs.
Applications:
  • Western Blot (WB) analysis of Recombinant protein using MYC-tag Monoclonal antibody (66004-1-Ig)

    WB analysis of Recombinant protein using 66004-1-Ig

    Western Blot of Myc-tagged fusion protein with mouse anti-Myc tag monoclonal antibody (66004-1-Ig) at various dilutions incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Transfected HEK-293 cells using MYC-tag Monoclonal antibody (66004-1-Ig)

    IP experiment of Transfected HEK-293 using 66004-1-Ig

    IP Result of anti-MYC-tag (IP:66004-1-Ig, 3ug; Detection:66004-1-Ig 1:5000) with Transfected HEK-293 cells lysate 500ug.

  • Immunofluorescence (IF) / fluorescent staining of Transfected HEK-293 cells using MYC-tag Monoclonal antibody (66004-1-Ig)

    IF Staining of Transfected HEK-293 using 66004-1-Ig

    Immunofluorescent analysis of Transfected HEK-293 cells using 66004-1-Ig (MYC-tag antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).


Tested Applications

Positive WB detected inRecombinant protein
Positive IP detected inTransfected HEK-293 cells
Positive IF detected inTransfected HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:20000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:2000-1:20000 for WB
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

66004-1-Ig targets MYC-tag in WB, IP, IF, CoIP, ELISA applications and shows reactivity with Recombinant protein samples.

Tested Reactivity Recombinant protein
Cited Reactivityhuman, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name MYC-tag
Gene symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The Myc tag corresponds to amino acid residues(EQKLISEEDL) of the human c-Myc protein. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. Myc-Tag mouse mAb detects recombinant proteins containing the Myc tag. The antibody recognizes the Myc-tag EQKLISEEDL fused to either the amino- or carboxy- terminus of targeted proteins.

Protocols

Product Specific Protocols
WB protocol for MYC-tag antibody 66004-1-IgDownload protocol
IF protocol for MYC-tag antibody 66004-1-IgDownload protocol
IP protocol for MYC-tag antibody 66004-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Cell

LECT2, a Ligand for Tie1, Plays a Crucial Role in Liver Fibrogenesis.

Authors - Meng Xu

Mol Cell

PTPS Facilitates Compartmentalized LTBP1 S-Nitrosylation and Promotes Tumor Growth under Hypoxia.

Authors - Qin Zhao

Circ Res

Yes-Associated Protein Promotes Angiogenesis Via Signal Transducer and Activator of Transcription 3 in Endothelial Cells.

Authors - Jinlong He

Nat Commun

The ubiquitin ligase UBR5 suppresses proteostasis collapse in pluripotent stem cells from Huntington's disease patients.

Authors - Seda Koyuncu
WB

Nat Commun

PMID: 28654087

Authors - Liufeng Wu
WB

Cell Res

ERK kinase phosphorylates and destabilizes the tumor suppressor FBW7 in pancreatic cancer.

Authors - Shunrong Ji