MYC-tag Monoclonal antibody

MYC-tag Monoclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Recombinant protein and More (1)

Applications

WB, IP, IF, CoIP,ELISA

Conjugate

Unconjugated

CloneNo.

2D11A8

Cat no : 66004-1-Ig

Synonyms


"MYC-tag Antibodies" Comparison

View side-by-side comparison of MYC-tag antibodies from other vendors to find the one that best suits your research needs.
  • WB analysis of Ag19172 using 66004-1-Ig

    WB analysis of Ag19172 using 66004-1-Ig

    Western Blot of Myc-tagged fusion protein with mouse anti-Myc tag monoclonal antibody (66004-1-Ig) at various dilutions incubated at room temperature for 1.5 hours.

  • IP experiment of Transfected HEK-293 using 66004-1-Ig

    IP experiment of Transfected HEK-293 using 66004-1-Ig

    IP Result of anti-MYC-tag (IP:66004-1-Ig, 3ug; Detection:66004-1-Ig 1:5000) with Transfected HEK-293 cells lysate 500ug.

  • IF Staining of Transfected HEK-293 using 66004-1-Ig

    IF Staining of Transfected HEK-293 using 66004-1-Ig

    Immunofluorescent analysis of Transfected HEK-293 cells using 66004-1-Ig (MYC-tag antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).


Tested Applications

Positive WB detected inRecombinant protein
Positive IP detected inTransfected HEK-293 cells
Positive IF detected inTransfected HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:20000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:2000-1:20000 for WB
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

66004-1-Ig targets MYC-tag in WB, IP, IF, CoIP,ELISA applications and shows reactivity with Recombinant protein samples.

Tested Reactivity Recombinant protein
Cited Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name MYC-tag
Gene symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The Myc tag corresponds to amino acid residues(EQKLISEEDL) of the human c-Myc protein. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. Myc-Tag mouse mAb detects recombinant proteins containing the Myc tag. The antibody recognizes the Myc-tag EQKLISEEDL fused to either the amino- or carboxy- terminus of targeted proteins.

Protocols

Product Specific Protocols
WB protocol for MYC-tag antibody 66004-1-IgDownload protocol
IF protocol for MYC-tag antibody 66004-1-IgDownload protocol
IP protocol for MYC-tag antibody 66004-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Leuk Lymphoma

Loc108167440 suppressed myeloma cell growth by P53-mediated apoptosis.

Authors - Bing Zhai

Cell Death Dis

PMID: 31024010

Authors - Dong-Ming Wu
humanWB

ACS Infect Dis

Resveratrol Attenuates Meningitic Escherichia coli-Mediated Blood-Brain Barrier Disruption.

Authors - Ruicheng Yang

Int J Ophthalmol

Soluble expression of recomb inant cMyc, Klf4, Oct4, and Sox2 proteins in bacteria and transduction into living cells.

Authors - Guo-Dan Liu
WB

Acta Biochim Biophys Sin (Shanghai)

Protein kinase Cα inhibits myocardin-induced cardiomyocyte hypertrophy through the promotion of myocardin phosphorylation.

Authors - Weizong Li
WB

Virol J

Herpes simplex virus 1 UL41 protein abrogates the antiviral activity of hZAP by degrading its mRNA.

Authors - Chenhe Su