Tested Applications
| Positive WB detected in | NR8383 cells, RAW 264.7 cells, mouse liver tissue, rat liver tissue |
| Positive IHC detected in | mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
29946-1-AP targets MYD88 in WB, IHC, IF, ELISA applications and shows reactivity with mouse, rat samples.
| Tested Reactivity | mouse, rat |
| Cited Reactivity | mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag30662 Product name: Recombinant mouse MYD88 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 50-150 aa of NM_010851 Sequence: LAEEMGFEYLEIRELETRPDPTRSLLDAWQGRSGASVGRLLELLALLDREDILKELKSRIEEDCQKYLGKQQNQESEKPLQVARVESSVPQTKELGGITTL Predict reactive species |
| Full Name | myeloid differentiation primary response gene 88 |
| Calculated Molecular Weight | 34 kDa |
| Observed Molecular Weight | 29-34 kDa |
| GenBank Accession Number | NM_010851 |
| Gene Symbol | Myd88 |
| Gene ID (NCBI) | 17874 |
| RRID | AB_3076471 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P22366 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MYD88 is a cytosolic adapter protein that plays a central role in the innate and adaptive immune response. MYD88 is the key adaptor protein for the interleukin-1 receptor (IL-1R) and most Toll-like receptors (TLRs), which are essential for innate immunity and pathogen-associated molecular pattern recognition. Mutations in the MyD88 gene lead to the development of cancer in humans and mice suggesting that MyD88 also plays a cell autonomous role in tissue homeostasis. MYD88 is detected in bone marrow.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for MYD88 antibody 29946-1-AP | Download protocol |
| WB protocol for MYD88 antibody 29946-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
MYD88 Polyclonal antibody (Cat# 29946-1-AP) has accumulated 16 citations published in journals such as J Control Release, Phytomedicine, Advanced Healthcare Materials, European Journal of Pharmacology, International Immunopharmacology, International Journal of Molecular Sciences, Frontiers in Pharmacology, and Food Research International. The associated research fields include TLR4/NF-κB immune signaling, inflammation, gut microbiota modulation, neuroinflammation, pulmonary fibrosis, cancer, autoimmune disease, sepsis, and intestinal barrier function.
| Species | Application | Title |
|---|---|---|
Phytomedicine Multiomics combined analysis reveals protective effect of 7-O-α-L-rhamnopyranosyl-kaempferol-3-O-β-D-glucopyranoside on autoimmune hepatitis | ||
Adv Healthc Mater Colon-Targeted Ginseng Polysaccharides-Based Microspheres for Improving Ulcerative Colitis via Anti-Inflammation and Gut Microbiota Modulation | ||
Chin Med Exploring the role of RCEO in macrophage-mediated modulation of pulmonary fibrosis. | ||
J Ethnopharmacol Prophylactic isoimperatorin protects against LPS-ALI via mTOR-dependent control of inflammation, oxidative stress and ferroptosis markers. | ||
J Agric Food Chem Panax ginseng Extract Alleviates Qi Deficiency Liver Cancer via the Gut-Liver Axis: Insights from Metabolomics and Microbiome. |
