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  • KD/KO Validated

MYL9 Polyclonal antibody

MYL9 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 15354-1-AP


20 kDa myosin light chain, LC20, MLC 2C, MLC2, MRLC1, MYL9, Myosin RLC, MYRL2

Tested Applications

Positive WB detected inmouse colon tissue, Caco-2 cells, rat colon tissue
Positive IHC detected inhuman liver cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells, C2C12 cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:5000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15354-1-AP targets MYL9 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MYL9 fusion protein Ag7600
Full Name myosin, light chain 9, regulatory
Calculated Molecular Weight 20 kDa
Observed Molecular Weight 20 kDa
GenBank Accession NumberBC002648
Gene Symbol MYL9
Gene ID (NCBI) 10398
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Myosin regulatory light polypeptide 9 (MYL9), also known as MLC2, belongs to the myosin regulatory subunits. It plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation at Thr19 and Ser20. Implicated in cytokinesis, receptor capping, and cell locomotion (PMID:11942626, PMID:2526655). Some studies have demonstrated that MYL9 may play important roles in various human cancers. The expression and phosphorylation of MYL9 (Thr19/Ser20) may be increased in human breast (PMID: 22144583) and liver cancers (PMID: 18648664), while decreased in human colon (PMID: 22752057) and bladder cancers (PMID: 21139803). MYL9 was the only gene differentially expressed in the aged versus young injured arteries in the rat smooth muscle cell layers (PMID:22003410).


Product Specific Protocols
WB protocol for MYL9 antibody 15354-1-APDownload protocol
IHC protocol for MYL9 antibody 15354-1-APDownload protocol
IF protocol for MYL9 antibody 15354-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



J Biol Chem

Brain-Derived Neurotrophic Factor regulates LYN kinase mediated Myosin Light Chain Kinase activation to modulate non-muscle myosin II activity in hippocampal neurons.

Authors - Xiao-Bing Li

Sci Rep

Proteomic profiling of concurrently isolated primary microvascular endothelial cells, pericytes, and vascular smooth muscle cells from adult mouse heart.

Authors - Zhiping Cao

Front Med (Lausanne)

Depiction of Aging-Based Molecular Phenotypes With Diverse Clinical Prognosis and Immunological Features in Gastric Cancer.

Authors - Fang He
  • KD Validated

Oncol Rep

Fenretinide inhibits the proliferation and migration of human liver cancer HepG2 cells by downregulating the activation of myosin light chain kinase through the p38‑MAPK signaling pathway.

Authors - Ling Zhang

JCI Insight

Transient expansion and myofibroblast conversion of adipogenic lineage precursors mediate bone marrow repair after radiation.

Authors - Leilei Zhong

Front Cell Dev Biol

Paracrine HB-EGF signaling reduce enhanced contractile and energetic state of activated decidual fibroblasts by rebalancing SRF-MRTF-TCF transcriptional axis

Authors - Junaid Afzal


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Irem (Verified Customer) (05-18-2022)

20 kDa band can be observed but there are other unspesific bands. Marker: Pageruler protein prestained marker

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Jurkat cells
MYL9 Antibody Western Blot validation (1:1000 dilution) in Jurkat cells (Cat no:15354-1-AP)