MYL9 Polyclonal antibody

MYL9 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15354-1-AP

Synonyms

20 kDa myosin light chain, LC20, MLC 2C, MLC2, MRLC1, MYL9, Myosin RLC, MYRL2



Tested Applications

Positive WB detected inmouse colon tissue, human colon tissue, Caco-2 cells
Positive IHC detected inhuman liver cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:5000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

15354-1-AP targets MYL9 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MYL9 fusion protein Ag7600
Full Name myosin, light chain 9, regulatory
Calculated molecular weight 20 kDa
Observed molecular weight 20 kDa
GenBank accession numberBC002648
Gene symbol MYL9
Gene ID (NCBI) 10398
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MYL9 antibody 15354-1-APDownload protocol
IHC protocol for MYL9 antibody 15354-1-APDownload protocol
IF protocol for MYL9 antibody 15354-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

JCI Insight

Transient expansion and myofibroblast conversion of adipogenic lineage precursors mediate bone marrow repair after radiation.

Authors - Leilei Zhong
humanWB

Front Med (Lausanne)

Depiction of Aging-Based Molecular Phenotypes With Diverse Clinical Prognosis and Immunological Features in Gastric Cancer.

Authors - Fang He
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Irem (Verified Customer) (05-18-2022)

20 kDa band can be observed but there are other unspesific bands. Marker: Pageruler protein prestained marker

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Jurkat cells
MYL9 Antibody Western Blot validation (1:1000 dilution) in Jurkat cells (Cat no:15354-1-AP)