|Positive WB detected in||human skeletal muscle tissue|
|Western Blot (WB)||WB : 1:500-1:2000|
|Sample-dependent, check data in validation data gallery|
66214-1-Ig targets MYOD1 in WB applications and shows reactivity with human, mouse, rat, pig samples.
|Tested Reactivity||human, mouse, rat, pig|
|Cited Reactivity||human, mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||MYOD1 fusion protein Ag13512|
|Full Name||myogenic differentiation 1|
|Calculated molecular weight||320 aa, 35 kDa|
|Observed molecular weight||47 kDa|
|GenBank accession number||BC064493|
|Gene ID (NCBI)||4654|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
MYOD1, also named as BHLHC1 or MYF3, is a 320 amino acid protein, which promotes the transcriptional activity of MYOD1 through its CDK9-mediated phosphorylation. This phosphorylation promotes its function in muscle differentiation. MYOD1 acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. MYOD1 together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. he calculated molecular weight of MYOD1 is 34 kDa, but modified MYOD1 is about 45-47 kDa. (PMID: 12037670)
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