Mouse Mmp9 Polyclonal antibody, PBS Only

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  WB analysis using 30592-1-AP (same clone as 30592-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30592-1-AP (Mmp9 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30592-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse lung using 30592-1-AP (same clone as 30592-1-PBS)

  IP result of anti-Mmp9 (IP:30592-1-AP, 4ug; Detection:30592-1-AP 1:6000) with mouse lung tissue lysate 1200 ug. This data was developed using the same antibody clone with 30592-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 30592-1-AP (same clone as 30592-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Mmp9 antibody (30592-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30592-1-PBS in a different storage buffer formulation.

• 

  IF Staining of U2OS using 30592-1-AP (same clone as 30592-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using Mmp9 antibody (30592-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30592-1-PBS in a different storage buffer formulation.

Mmp9 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Cat No. 30592-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, IP, Indirect ELISA

Synonyms

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Product Information

30592-1-PBS targets Mmp9 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: matrix metallopeptidase 9
• Calculated Molecular Weight: 81kd
• Observed Molecular Weight: 80-100 kDa
• GenBank Accession Number: NM_013599
• Gene Symbol: Mmp9
• Gene ID (NCBI): 17395
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P41245-1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Mmp9 (matrix metallopeptidase 9), also known as Clg4b. It is expected to be located inextracellular matrix. Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, tissue remodeling, and disease processes, such as arthritis or metastasis. Studies in rhesus monkeys suggest that MMP9 is involved in IL-8-induced mobilization hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. The calculated molecular weight of Mmp9 is 80 kDa, the pro-MMP9 is 92 kDa, and this protein can exist as a dimer of 180 kDa (PMID:7492685).