Mmp9 Polyclonal antibody

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• Mmp9 Polyclonal antibody (30592-1-AP)

Mmp9 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 30592-1-AP Copied!

Publications (29)

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Host / Isotype

Rabbit / IgG

Reactivity

mouse, human and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

AW743869, B/MMP9, Clg4b, matrix metallopeptidase 9, MMP 9, Mmp9, pro MMP 9

• 

  WB analysis using 30592-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30592-1-AP (Mmp9 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of mouse lung using 30592-1-AP

  IP result of anti-Mmp9 (IP:30592-1-AP, 4ug; Detection:30592-1-AP 1:6000) with mouse lung tissue lysate 1200 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of A431 using 30592-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Mmp9 antibody (30592-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of U2OS using 30592-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using Mmp9 antibody (30592-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse lung tissue, mouse spleen tissue
• Positive IP detected in: mouse lung tissue
• Positive IF/ICC detected in: U2OS cells, A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30592-1-AP targets Mmp9 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with mouse, human samples.

• Tested Reactivity: mouse, human
• Cited Reactivity: human, mouse, rat, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: matrix metallopeptidase 9
• Calculated Molecular Weight: 81kd
• Observed Molecular Weight: 80-100 kDa
• GenBank Accession Number: NM_013599
• Gene Symbol: Mmp9
• Gene ID (NCBI): 17395
• RRID: AB_3086370
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P41245-1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mmp9 (matrix metallopeptidase 9), also known as Clg4b. It is expected to be located inextracellular matrix. Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, tissue remodeling, and disease processes, such as arthritis or metastasis. Studies in rhesus monkeys suggest that MMP9 is involved in IL-8-induced mobilization hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. The calculated molecular weight of Mmp9 is 80 kDa, the pro-MMP9 is 92 kDa, and this protein can exist as a dimer of 180 kDa (PMID:7492685).

Publications

What published studies show

The MMP9 Polyclonal antibody (Cat# 30592-1-AP) has 29 citations. These appear in journals including ACS Nano, Advanced Science, Small, Acta Biomaterialia, Oncogene, Phytomedicine, International Journal of Molecular Sciences, Scientific Reports, Cell Signaling, and Frontiers in Pharmacology. Research fields span cancer (prostate, pancreatic, ovarian, osteosarcoma), osteoporosis, osteoarthritis, wound healing, skin photoaging, stroke, liver fibrosis, atherosclerosis, inflammatory bowel disease, and sepsis, with most studies using Western blot.

Automatically generated from the 29 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	ACS Nano; Highly Efficient Intrastromal Dual-Drug Delivery by a Dissolving Bilayer Microneedle for Synergistic Therapy against Corneal Neovascularization.; Authors - Zhaoliang Zhang
mouse	WB	J Nanobiotechnology; Lacc1-engineered extracellular vesicles reprogram mitochondrial metabolism to alleviate inflammation and cartilage degeneration in TMJ osteoarthritis; Authors - Xiaofeng Hu
		Adv Sci (Weinh); HD-tDCS Restores Perivascular AQP4 Polarization via PPARγ Modulation to Enhance Glymphatic Clearance After Intracerebral Hemorrhage in Mice.; Authors - Zhiming Li
mouse	WB,IF	Small; A Constant Filgotinib Delivery Adhesive Platform Based on Polyethylene Glycol (PEG) Hydrogel for Accelerating Wound Healing via Restoring Macrophage Mitochondrial Homeostasis; Authors - Jian Xie
mouse	WB	Phytomedicine; A novel METTL3 inhibitor nimbolide ameliorates osteoporosis via orchestrating osteoclastogenesis in an m6A-dependent manner.; Authors - Chenhui Cai
mouse	WB	Adv Healthc Mater; Zinc-Containing Bioactive Glass Programs Macrophage Polarization through Extracellular Traps Regulation for Enhanced Diabetic Wound Healing.; Authors - RuiYang Sun