Mmp9 Polyclonal antibody

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• Mmp9 Polyclonal antibody (30592-1-AP)

Mmp9 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 30592-1-AP

Publications(28)

Host / Isotype

Rabbit / IgG

Reactivity

mouse, human and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

AW743869, B/MMP9, Clg4b, matrix metallopeptidase 9, MMP 9, Mmp9, pro MMP 9

• 

  WB analysis using 30592-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30592-1-AP (Mmp9 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of mouse lung using 30592-1-AP

  IP result of anti-Mmp9 (IP:30592-1-AP, 4ug; Detection:30592-1-AP 1:6000) with mouse lung tissue lysate 1200 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of A431 using 30592-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Mmp9 antibody (30592-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of U2OS using 30592-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using Mmp9 antibody (30592-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse lung tissue, mouse spleen tissue
• Positive IP detected in: mouse lung tissue
• Positive IF/ICC detected in: U2OS cells, A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30592-1-AP targets Mmp9 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with mouse, human samples.

• Tested Reactivity: mouse, human
• Cited Reactivity: human, mouse, rat, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: matrix metallopeptidase 9
• Calculated Molecular Weight: 81kd
• Observed Molecular Weight: 80-100 kDa
• GenBank Accession Number: NM_013599
• Gene Symbol: Mmp9
• Gene ID (NCBI): 17395
• RRID: AB_3086370
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P41245-1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mmp9 (matrix metallopeptidase 9), also known as Clg4b. It is expected to be located inextracellular matrix. Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, tissue remodeling, and disease processes, such as arthritis or metastasis. Studies in rhesus monkeys suggest that MMP9 is involved in IL-8-induced mobilization hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. The calculated molecular weight of Mmp9 is 80 kDa, the pro-MMP9 is 92 kDa, and this protein can exist as a dimer of 180 kDa (PMID:7492685).

Publications

What published studies show

Automatically generated from the publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB,IF	Small; A Constant Filgotinib Delivery Adhesive Platform Based on Polyethylene Glycol (PEG) Hydrogel for Accelerating Wound Healing via Restoring Macrophage Mitochondrial Homeostasis; Authors - Jian Xie
mouse	WB	Adv Healthc Mater; Zinc-Containing Bioactive Glass Programs Macrophage Polarization through Extracellular Traps Regulation for Enhanced Diabetic Wound Healing.; Authors - RuiYang Sun
	WB	Gene; SOGA1 drives ovarian cancer progression via regulation of GNAI1 and activation of the TNF/NF-κB pathway; Authors - Tengfei Long
mouse	IF	Exp Eye Res; Proanthocyanidins inhibit the inflammation response of dry eye by regulating IRAK4/JAK1/STAT1 signaling pathway.; Authors - Ying Lian
	WB	Ecotoxicol Environ Saf; Peptide CyRL-QN15 alleviates UVB-induced skin aging by promoting the repair of barrier; Authors - Zijian Kang
rat	WB	Front Toxicol; Tributyl phosphate as a type of environmental endocrine disruptor associated with liver fibrosis: insights from NHANES and in vitro validation; Authors - Liwei Yang