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Recombinant Mouse Adiponectin protein (His Tag)

Species

Mouse

Purity

>90 %, SDS-PAGE

Tag

His Tag

Activity

not tested

Cat no : Eg0262

Validation Data Gallery

  • Purity of Recombinant Mouse Adiponectin was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse Adiponectin was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression CHO-derived Mouse Adiponectin protein Glu18-Asn247 (Accession# Q60994) with a His tag at the C-terminus.
GeneID 11450
Accession Q60994
PredictedSize 28.7 kDa
SDS-PAGE 30-34 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Adiponectin, also known as Acrp30, AdipoQ, and GBP28, is an adipocyte-secreted protein that circulates in high concentrations in the serum and acts to increase insulin sensitivity. Adiponectin modulates a number of metabolic processes, including glucose regulation and fatty acid oxidation. The serum concentration of adiponectin decreases in obese patients, opposite to other adipocytokines. In recent studies, serum adiponectin level progressively decreased in patients according to the severity of obesity, insulin resistance, diabetes mellitus and cardiovascular diseases. Low serum adiponectin level may be a predictive factor for type 2 diabetes mellitus (T2DM) and other cardiovascular diseases.

References:

1. Wang ZV. et al. (2016) J Mol Cell Biol. 8(2):93-100.
2. Liu M. et al. (2014) Best Pract Res Clin Endocrinol Metab.28(1):25-31.
3. Díez JJ. et al. (2003) Eur J Endocrinol.148(3):293-300.
4. Yamauchi T. et al. (2003) Nature.423(6941):762-9.