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Recombinant Mouse CD117/c-kit protein (His Tag)

Species

Mouse

Purity

>95 %, SDS-PAGE

Tag

His Tag

Activity

EC50: 2-8 ng/mL

Cat no : Eg0883

Validation Data Gallery

  • Purity of Recombinant Mouse CD117 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse CD117 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • Immobilized Mouse CD117 (His tag) at 1 μg/mL (100 μL/well) can bind Mouse SCF (His tag) with a linear range of 2-8 ng/mL.

    Immobilized Mouse CD117 (His tag) at 1 μg/mL (100 μL/well) can bind Mouse SCF (His tag) with a linear range of 2-8 ng/mL.

Product Information

Purity >95 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Immobilized Mouse CD117 (His tag) at 1 μg/mL (100 μL/well) can bind Mouse SCF (His tag) with a linear range of 2-8 ng/mL.
Expression HEK293-derived Mouse CD117 protein Ser25-Pro527 (Accession# P05532-1) with a His tag at the C-terminus.
GeneID 16590
Accession P05532-1
PredictedSize 56.8 kDa
SDS-PAGE 65-85 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

CD117, also known as c-Kit and SCFR, is a transmembrane protein with tyrosine kinase activity encoded by the oncogene c-kit. It is a member of the type III receptor tyrosine kinase family, which also includes CSF-1R, PDGFRβ, PDGFRα, and FLT3. CD117 is expressed on hematopoietic stem cells and progenitor cells, mast cells, and is also found in a wide range of non-haemopoietic cell types (including melanocytes, germ cells, astrocytes, renal tubules, breast glandular epithelial cells, sweat glands, and interstitial cells of Cajal). CD117 plays an important role in early haemopoiesis. It is also involved in pigmentation, fertility, gut movement, and some aspects of the nervous system.

References:

1.Y Yarden, et al. (1987) EMBO J. 6(11):3341-51.
2.L K Ashman (1999) Int J Biochem Cell Biol. 31(10):1037-51.
3.Johan Lennartsson (2012) Physiol Rev. 92(4):1619-49. 
4.Brittni M Foster, et al. (2018) Biomedicines. 6(1):31.