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Recombinant Mouse CD2 protein (rFc Tag) (HPLC verified)

Species

Mouse

Purity

>90 %, SDS-PAGE
>90 %, SEC-HPLC

Tag

rFc Tag

Activity

not tested

Cat no : Eg1403

Validation Data Gallery

  • Purity of Recombinant Mouse CD2 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse CD2 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • The purity of Mouse CD2 was greater than 90% as determined by SEC-HPLC.

    The purity of Mouse CD2 was greater than 90% as determined by SEC-HPLC.

Product Information

Purity >90 %, SDS-PAGE
>90 %, SEC-HPLC
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Mouse CD2 protein Arg23-Ser203 (Accession# P08920) with a rabbit IgG Fc tag at the C-terminus.
GeneID 12481
Accession P08920
PredictedSize 47.0 kDa
SDS-PAGE 52-70 kDa, reducing (R) condition
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

CD2 is a cell surface glycoprotein present on a majority of thymocytes. The CD2 antigen was identified as one of the earliest T cell markers and was shown to be important for thymocyte development. Notably, only a minor percentage of B cells express CD2 in humans while CD2 is broadly expressed on murine B cells. CD2 is a transmembrane glycoprotein of the immunoglobulin superfamily expressed on the surface of T cells, NK cells, thymocytes and dendritic cells. The glycoprotein CD2 is a costimulatory receptor expressed mainly on T and NK cells that binds to LFA3.

References:

1.Haynes BF, et al. (1988) J Immunol. 141(11):3776-84.
2.Vollger LW, et al. (1987) J Immunol. 138(2):358-63.
3.Kingma DW, et al. (2002) Cytometry. 50(5):243-8.
4.Yagita H, et al. (1989) Eur J Immunol. 19(12):2211-7.
5.Krensky AM, et al. (1983) J Immunol. 131(2):611-6.
6.Matsui T, et al. (2009) J Immunol. 182(11):6815-23.
7.Binder C, et al. (2020) Front Immunol. 11:1090.