Recombinant Mouse CD200R1 protein (His tag)

Species

Mouse

Purity

>95 %, SDS-PAGE

Tag

His tag

Activity

EC50: 5-19 ng/mL

Cat no : Eg0988

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Validation Data Gallery

• 

  Purity of Recombinant Mouse CD200R1 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

• 

  Immobilized Mouse CD200R1 (His tag) at 1 μg/mL (100 μL/well) can bind Mouse CD200 (hFc tag) with a linear range of 5-19 ng/mL.

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Product Information

• Purity: >95 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Immobilized Mouse CD200R1 (His tag) at 1 μg/mL (100 μL/well) can bind Mouse CD200 (hFc tag) with a linear range of 5-19 ng/mL.

• Expression: HEK293-derived Mouse CD200R1 protein Thr26-Pro238 (Accession# Q9ES57) with a His tag at the C-terminus.
• GeneID: 57781
• Accession: Q9ES57
• PredictedSize: 23.9 kDa
• SDS-PAGE: 40-55 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Cell surface glycoprotein CD200 receptor 1 (CD200R1), also named as CD200R, CRTR2, MOX2R and OX2R, belongs to the CD200R family. It is a type I transmembrane glycoprotein containing two Ig-like domains in the extracellular region, a transmembrane and a cytoplasmic domain. CD200R1 is an inhibitory receptor for the CD200/OX2 cell surface glycoprotein. CD200 is expressed on a variety of cells of hematopoietic and non-hematopoietic origin, while CD200R1 is primarily expressed by myeloid and T cell lineage. The CD200-CD200R interaction plays a role in immunosuppression and suppression of anti-tumor immune responses.

References:

1. Gavin J Wright, et al. (2003). J Immunol. 171(6):3034-46. 
2. Reginald Gorczynski, et al. (2004). J Immunol. 172(12):7744-9.
3. T P Rygiel, et al. (2012). Oncogene. 31(24):2979-88.