Recombinant Mouse CD83 protein (rFc Tag)

Species

Mouse

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg1387

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Validation Data Gallery

• 

  Purity of Recombinant Mouse CD83 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Mouse CD83 protein Met22-Arg133 (Accession# O88324) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 12522
• Accession: O88324
• PredictedSize: 38.6 kDa
• SDS-PAGE: 42-55 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

CD83 is a member of the immunoglobulin (Ig) superfamily, consisting of an extracellular V-type Ig-like domain, a transmembrane domain, and a cytoplasmic tail. CD83 is one of the most prominent markers for fully matured dendritic cells (DCs). It is also found on the surface of other activated hematopoietic cells, including lymphocytes, monocytes, macrophages, and neutrophils. CD83 regulates the maturation, activation, and homeostasis of numerous immune cells. CD83 can also expressed as a soluble form. Soluble CD83 can bind to DCs and inhibit their maturation.

References:

1. Prechtel AT. et al. (2007). Arch Dermatol Res. 299(2):59-69.
2. Li Z. et al. (2019). Front Immunol. 10:1312.
3. Grosche L. et al. (2020). Front Immunol. 11:721.
4. Lechmann M. et al. (2002). Int Arch Allergy Immunol. 129(2):113-8.