Recombinant Mouse Cathepsin B protein (mFc Tag)

Species

Mouse

Purity

>90 %, SDS-PAGE

Tag

mFc Tag

Activity

not tested

Cat no : Eg3937

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Validation Data Gallery

• 

  Purity of Recombinant Mouse Cathepsin B was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Mouse Cathepsin B protein His18-Phe339 (Accession# P10605) with a mouse IgG Fc tag at the C-terminus.
• GeneID: 13030
• Accession: P10605
• PredictedSize: 62.0 kDa
• SDS-PAGE: 28 kDa, 42-47 kDa and 65-80 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

CTSB(Cathepsin B) is also named CPSB and belongs to the peptidase C1 family. It participates in intracellular degradation and turnover of proteins. Cathepsin B precursors found in human malignant ascites fluid do not possess mannose-rich carbohydrates, suggesting a defect in the post-translational processing of carbohydrate moieties on tumors. Cathepsin B exists in both glycosylated and unglycosylated forms. Western blot can detect the 43 kDa form of pro-CTSB, 31 kDa, and 25 kDa mature forms in mouse brain.

References:

1. Moin K. et al. (1992). Biochem J. 285 (Pt 2)(Pt 2):427-434.
2. Vitner EB. et al. (2010). Hum Mol Genet. 19(18):3583-3590.