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Recombinant Mouse CX3CL1 protein (His Tag)

Species

Mouse

Purity

>90 %, SDS-PAGE

Tag

His Tag

Activity

not tested

Cat no : Eg0704

Validation Data Gallery

  • Purity of Recombinant Mouse CX3CL1 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse CX3CL1 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Mouse CX3CL1 protein Gln25-Arg337 (Accession# O35188) with a His tag at the C-terminus.
GeneID 20312
Accession O35188
PredictedSize 34 kDa
SDS-PAGE 60-85 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

CX3CL1, also known as fractalkine, is a trans-membrane chemokine that acts as a ligand for both CX3CR1 and integrins αvβ3 and α4β1. CX3CL1 is expressed by many hematopoietic and nonhematopoietic cells, including macrophages, endothelial cells, neurons, and fibroblasts. It is involved in immune responses and inflammation. CX3CL1 exists in a membrane-anchored molecule and a soluble form, generated by proteolytic cleavage.

References:

1. D L Rossi, et al. (1998) Genomics. 47(2):163-70.
2. K J Garton, et al. (2001) J Biol Chem. 276(41):37993-8001. 
3. Christian Hundhausen, et al. (2003) Blood. 102(4):1186-95.
4. Brian A Jones, et al. (2010) Mol Interv. 10(5):263-70. 
5. Masaaki Fujita, et al. (2012) J Immunol. 189(12):5809-19.