Recombinant Mouse DPP4/CD26 protein (His Tag)

Species

Mouse

Purity

>90 %, SDS-PAGE

Tag

His Tag

Activity

not tested

Cat no : Eg1309

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Validation Data Gallery

• 

  Purity of Recombinant Mouse DPP4 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Mouse DPP4 protein Ser29-His760 (Accession# Accession P28843) with a His tag at the C-terminus.
• GeneID: 13482
• Accession: P28843
• PredictedSize: 85.6 kDa
• SDS-PAGE: 85-110 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Dipeptidyl peptidase 4 (DPP4, also known as CD26) is a serine exopeptidase that cleaves X-proline dipeptides from the N terminus of polypeptides. It is a type II membrane protein consisting of a short cytoplasmic tail, a transmembrane domain, and a large extracellular domain comprised of a flexible stalk, glycosylation-rich region, cysteine-rich region and catalytic region. DPP4 is widely expressed on T cells, activated B cells, activated NK cells and myeloid cells as well as on epithelial, endothelial cells of a variety of tissues. A soluble form of DPP4 lacking the intracellular part and the transmembrane region is found in body fluids. Altered levels of serum soluble DPP4 have been reported in many diseases including inflammatory and infectious diseases, and tumors.

References:

1. Anne-Marie Lambeir. et al. (2003). Crit Rev Clin Lab Sci. 40(3):209-94.
2. Oscar J Cordero. et al. (2009). Cancer Immunol Immunother. 58(11):1723-47.
3. C Klemann. et al. (2016). Clin Exp Immunol. 185(1):1-21.