Speedy™ Mouse E-Cadherin One-Step ELISA Kit
Cat no : SE60052
Synonyms
cadherin 1, Cdh1, Ecad, L CAM, Um
Validation Data Gallery
Product Information
| Product name | Speedy™ Mouse E-Cadherin One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Tissue lysate |
| Assay type | Sandwich |
| Sensitivity | 1.9 pg/mL |
| Range | 18.8-1200 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 12550 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 94% | 86%-107% |
| Tissue lysate | 87% | 81%-95% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 648.2 | 23.4 | 3.6 |
| 2 | 8 | 158.1 | 2.7 | 1.7 |
| 3 | 8 | 82.8 | 1.7 | 2.1 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 656.6 | 22.8 | 3.5 |
| 2 | 16 | 160.6 | 4.8 | 3.0 |
| 3 | 16 | 81.5 | 2.3 | 2.8 |
Background Information
E-cadherin is a transmembrane glycoprotein that connects epithelial cells at adherens junctions. In normal cells, E-cadherin exerts its tumor-suppressing role mainly by sequestering β-catenin from its binding to LEF (Lymphoid enhancer factor)/TCF (T cell factor), which serves the function of transcribing genes of the proliferative Wnt signaling pathway. The loss of E-cadherin expression associated with the epithelial-mesenchymal transition (EMT) occurs frequently during tumor metastasis. Furthermore, Soluble E-cadherin may be biomarkers for the diagnosis and prognosis of cancer.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | cadherin 1, Cdh1, Ecad, L CAM, Um |