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Recombinant Mouse EGF protein (rFc Tag)

Species

Mouse

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg1965

Validation Data Gallery

  • Purity of Recombinant Mouse EGF was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse EGF was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Mouse EGF protein Asn977-Arg1029 (Accession# P01132) with a rabbit IgG Fc tag at the N-terminus.
GeneID 13645
Accession P01132
PredictedSize 33.2 kDa
SDS-PAGE 32-38 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Epidermal growth factor (EGF) is a member of the epidermal growth factor superfamily. EGF preproprotein is proteolytically processed to generate the 53-amino acid epidermal growth factor peptide. EGF binds to the EGF receptor on the surface of cells and mediates intrinsic phosphorylation of the receptor on tyrosine residues. It has been detected in nearly all body fluids, such as urine (urogastrone), saliva, milk and platelet-rich plasma. EGF plays important roles in multiple biological processes, such as the regulation of cell growth, proliferation, and differentiation.

References:

1. Gregory H.et al. (1985).J Cell Sci Suppl.3:11-7. 
2. Stroobant P.et al. (1985). Cell.42(1):383-93. 
3. Carpenter G.et al. (1986). Exp Cell Res.164(1):1-10. 
4. Derynck R.et al. (1986).J Cell Biochem.32(4):293-304. 
5. St-Arnaud R.et al. (1984). Biochimie.66(7-8):515-30.