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Recombinant Mouse Flt3 ligand protein (His Tag)

Species

Mouse

Purity

>90 %, SDS-PAGE

Tag

His Tag

Activity

not tested

Cat no : Eg0677

Validation Data Gallery

  • Purity of Recombinant Mouse Flt3 ligand was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse Flt3 ligand was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression CHO-derived Mouse Flt3 ligand protein Gly27-Arg188 (Accession# P49772-1) with a His tag at the C-terminus.
GeneID 14256
Accession P49772-1
PredictedSize 19.5 kDa
SDS-PAGE 17-25 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Flt-3 Ligand, also known as FLT3L, is a cytokine that is of paramount importance in the proliferation of primitive hematopoietic progenitors. By acting on its receptor Flt-3 (CD135) and synergizing with other cytokines, FLT3L plays a pivotal role in promoting myeloid and lymphoid progenitors proliferation and differentiation. Besides regulating the development of immune cells during steady-state conditions, FLT3L levels are raised upon inflammation. For example, increased serum levels of FLT3L have been observed during malaria in mice. Previous studies have shown that mice lacking FLT3L have deficient hematopoiesis function, resulting in reduced numbers of hematopoietic progenitor cells, dendritic cells, and natural killer cells.

References:

1. Solanilla A, et al. (2000). Leukemia. 14(1): 153-162
2. Ramos MI, et al. (2014). Autoimmun Rev. 13(2):117-124
3. Pierre Guermonprez, et al. (2013). Nat Med. 19(6): 730-738
4. Sara M. Parigi, et al. (2018). Scientific Reports. 8: 154
5. McKenna HJ, et al. (2000). Blood. 95(11): 3489-3497.