Recombinant Mouse Glycophorin A protein (His Tag)
Species
Mouse
Purity
>90 %, SDS-PAGE
Tag
C-His
Activity
not tested
Cat no : Eg1886
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Mouse Glycophorin A protein Met1-Val108 (Accession# P14220) with a His tag at the C-terminus. |
| GeneID | 14934 |
| Accession | P14220 |
| PredictedSize | 12.2 kDa |
| SDS-PAGE | kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
Glycophorin A (GYPA) is also named CD235a and PAS-2. It is a component of the ankyrin-1 complex, a multiprotein complex involved in the stability and shape of the erythrocyte membrane. GYPA is the major transmembrane sialoglycoprotein in erythrocytes. It is a dimeric type I transmembrane protein carrying 15 closely clustered O-linked tetrasaccharides capped with sialic acid/N-acetylneuraminic acid (Neu5Ac). This 36 kDa protein represents the major sialoglycoprotein of the red blood cell membrane displaying about one million copies per cell.
References:
1. Vallese F, et al. (2022). Nat Struct Mol Biol. 29(7):706-718. 2. Hassoun H, et al. (1998). Blood. 91(6):2146-51.