Recombinant Mouse IL-5RA/CD125 protein (hFc Tag)

Species

Mouse

Purity

>90 %, SDS-PAGE

Tag

hFc Tag

Activity

not tested

Cat no : Eg0810

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Validation Data Gallery

• 

  Purity of Recombinant Mouse IL-5RA was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Mouse IL-5RA protein Asp18-His339 (Accession# P21183) with a human IgG1 Fc tag at the C-terminus.
• GeneID: 16192
• Accession: P21183
• PredictedSize: 63.5 kDa
• SDS-PAGE: 65-75 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

IL-5RA, also known as CD125, is a crucial component of the interleukin-5 receptor complex, which plays a significant role in the immune system, particularly in the regulation of eosinophil activity. Eosinophils are a type of white blood cell that are primarily involved in the body's response to parasitic infections and are also significantly implicated in allergic reactions and asthma. The interaction between interleukin-5 (IL-5) and its receptor IL-5RA is vital for the survival, activation, and differentiation of eosinophils.

References:

1.Takaki S, et al. (1994). Mol Cell Biol. 14 (11):7404-13.
2.Barry SC, et al. (1991) Cytokine. 3(4):339-44. 
3.dLawrence MG, et al. (2022) Ann Allergy Asthma Immunol.128 (1):53-60.e3.