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Recombinant Mouse CD126/IL-6R alpha protein (His Tag)

Species

Mouse

Purity

>95 %, SDS-PAGE

Tag

His Tag

Activity

EC50: 50-200 ng/mL

Cat no : Eg0664

Validation Data Gallery

  • Purity of Recombinant Mouse IL-6R alpha was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse IL-6R alpha was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • Immobilized Mouse IL-6R alpha (His tag) at 1 μg/mL (100 μL/well) can bind Mouse IL-6 (Myc tag, His tag) with a linear range of 50-200 ng/mL.

    Immobilized Mouse IL-6R alpha (His tag) at 1 μg/mL (100 μL/well) can bind Mouse IL-6 (Myc tag, His tag) with a linear range of 50-200 ng/mL.

Product Information

Purity >95 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Immobilized Mouse IL-6R alpha (His tag) at 1 μg/mL (100 μL/well) can bind Mouse IL-6 (Myc tag, His tag) with a linear range of 50-200 ng/mL.
Expression HEK293-derived Mouse IL-6R alpha protein Leu20-Glu357 (Accession# P22272) with a His tag at the C-terminus.
GeneID 16194
Accession P22272
PredictedSize 38.8 kDa
SDS-PAGE 52-65 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Interleukin-6 (IL-6) is a pleiotropic cytokine produced by a variety of cells during infection, trauma, and immunological challenge. IL-6 acts via a receptor complex consisting of two distinct membrane-bound glycoproteins, an 80-kDa IL-6-binding subunit (IL-6R alpha, CD126, gp80) and a 130-kDa signal-transducing element (gp130, CD130). After binding of IL-6 to membrane-bound IL-6R alpha, the complex of IL-6 and IL-6R alpha associates with gp130, thus activating the receptor. Expression of gp130 is found in almost all organs while expression of IL-6R alpha is predominantly confined to hepatocytes and leukocyte subpopulations (monocytes, neutrophils, T cells, and B cells). Soluble form of IL-6R alpha has been found in human serum and urine.

References:

1. D Novick. (1989) J Exp Med. 170(4):1409-14. 
2. M Honda. (1992) J Immunol. 148(7):2175-80.
3. H Tilg, et al. (1994) Blood. 83(1):113-8.
4. P C Heinrich. (1998) Biochem J. 334(2):297-314. 
5. S A Jones. (2001) FASEB J. 15(1):43-58.