Recombinant Mouse IL-9 protein (rFc Tag)

Species

Mouse

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg3827

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Validation Data Gallery

• 

  Purity of Recombinant Mouse IL-9 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Mouse IL-9 protein Gln19-Pro144 (Accession# P15247) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 16198
• Accession: P15247
• PredictedSize: 40.4 kDa
• SDS-PAGE: 48-62 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 150 mM NaCl, pH 8.0. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Interleukin 9 (IL-9) is a γc-family cytokine that is highly produced by T-helper 9 (Th9) cells. IL-9 could promote the survival and activation of various cellular targets, including mast cells, B cells, T cells, and structural cells. Its expression is considered a hallmark of Th2-lineage cells. Primarily studied in Th2-type immunity, IL-9 was shown to be involved in asthma, allergy, and host defense against helminth infections. IL-9 also stimulates cell proliferation and prevents apoptosis. It functions through the interleukin 9 receptor (IL9R), which activates different signal transducer and activator (STAT) proteins and thus connects this cytokine to various biological processes.

References:

1. Ann S Heiman. et al. (2005)J Interferon Cytokine Res. 25(2):82-91.
2. Nicola L Diny. et al. (2016) Eur J Immunol. 46(12): 2749-2760.
3. Petronela Ancuta. et al. (2006) J Immunol. 176(10): 5760-71.
4. Witold Owczarek. et al. (2010) Cytokine. 50(2):181-5.