Mouse IgG1 isotype control Monoclonal antibody

Mouse IgG1 isotype control Monoclonal Antibody for FC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Megathura crenulata and More (1)

Applications

WB, RIP, IP, IHC, FC, CoIP, Cell treatment, ELISA

Conjugate

Unconjugated

Publications

8

CloneNo.

1F8D3

Cat no : 66360-1-Ig

Synonyms

Kappa light chain, Mouse IgG1, Mouse IgG1 isotype control

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• Validation Data Gallery
• Proteintech vs. Other Vendors

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  WB analysis using 66360-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66360-1-Ig (Mouse IgG1 isotype control antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66360-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66360-1-Ig (Mouse IgG1 isotype control antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of K-562 using 66360-1-Ig

  1X10^6 K-562 cells were stained with 0.20ug Mouse IgG1 isotype control antibody (66360-1-Ig, red) and another control antibody (blue). Fixed with 90% MeOH.

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Tested Applications

Positive WB detected in
Positive FC detected in

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Flow Cytometry (FC): FC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below
• RIP: See 1 publications below

Product Information

66360-1-Ig targets Mouse IgG1 isotype control in WB, RIP, IP, IHC, FC, CoIP, Cell treatment, ELISA applications and shows reactivity with Megathura crenulata samples.

• Tested Reactivity: Megathura crenulata
• Cited Reactivity: mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: Mouse IgG1 isotype control
• RRID: AB_2827991
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

This antibody is used as the isotype control of mouse IgG1 (Kappa). It can be used as the isotype control in Flow Cytometry, Immuno-Precipitation and other experiments. The immunogen of this antibody is Keyhole Limpet Hemocyanin (KLH).

Publications

Species	Application	Title
	CoIP	FASEB J; Bile acid restrained T cell activation explains cholestasis aggravated hepatitis B virus infection; Authors - Chujie Ding
	IP	Exp Cell Res; CBL aggravates Ang II-induced cardiac hypertrophy via the VHL/HIF-1α pathway.; Authors - Yuting Yang
	IHC	Am J Rhinol Allergy; Co-Expression and Localization of Angiotensin-Converting Enzyme-2 (ACE2) and the Transmembrane Serine Protease 2 (TMPRSS2) in Paranasal Ciliated Epithelium of Patients with Chronic Rhinosinusitis.; Authors - Tomohiro Kawasumi
	RIP	Cell Biosci; PABPN1 regulates mRNA alternative polyadenylation to inhibit bladder cancer progression; Authors - Liang Chen
		Stem Cell Res Ther; Menstrual blood-derived endometrial stem cells alleviate neuroinflammation by modulating M1/M2 polarization in cell and rat Parkinson's disease models; Authors - Han Li
	IP	Acta Biochim Biophys Sin (Shanghai); PGAM5 deacetylation mediated by SIRT2 facilitates lipid metabolism and liver cancer proliferation; Authors - Gongyu Fu