Mouse IgG1 isotype control Monoclonal antibody

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• Mouse IgG1 isotype control Monoclonal antibody (66360-1-Ig)

Mouse IgG1 isotype control Monoclonal Antibody for WB, ELISA

Cat No. 66360-1-Ig Copied!

Clone No. 1F8D3

Publications (40)

Download datasheet

Host / Isotype

Mouse / IgG1

Reactivity

megathura crenulata and More (2)

Applications

WB, IHC, IF, IP, CoIP, RIP, Neutralization, ELISA
For FC, select conjugated version.

Synonyms

IgG1 isotype control, Kappa light chain, Mouse IgG1

• Validation Data Gallery
• Proteintech vs. Other Vendors

• 

  WB analysis using 66360-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66360-1-Ig (Mouse IgG1 isotype control antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 66360-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66360-1-Ig (Mouse IgG1 isotype control antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

Positive WB detected in

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66360-1-Ig targets Mouse IgG1 isotype control in WB, IHC, IF, IP, CoIP, RIP, Neutralization, ELISA applications and shows reactivity with megathura crenulata samples.

• Tested Reactivity: megathura crenulata
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: Mouse IgG1 isotype control
• RRID: AB_2827991
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

This antibody is used as the isotype control of mouse IgG1 (Kappa). It can be used as the isotype control in Flow Cytometry, Immuno-Precipitation and other experiments. The immunogen of this antibody is Keyhole Limpet Hemocyanin (KLH).

Publications

What published studies show

Mouse IgG1 isotype control Monoclonal antibody (1F8D3), catalog number 66360-1-Ig, has 40 total citations. Citations appear in journals including Nature Communications, Nature Biomedical Engineering, Nature Cell Biology, Molecular Cell, Nature Chemical Biology, Advanced Science, Cell Reports, Cell Death & Disease, FASEB Journal, and Journal of Immunology. Research fields encompass oncology, immunology, virology, neuroscience, cardiology, metabolism, stem cell biology, reproductive biology, and epigenetics.

Automatically generated from the 40 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		Cancer Commun (Lond); m6A-modified EHD1 controls PD-L1 endosomal trafficking to modulate immune evasion and immunotherapy responses in lung adenocarcinoma; Authors - Fanglin Tian
		Nat Biomed Eng; Rapid and sensitive detection of cancer-derived small extracellular vesicles using Janus particles.; Authors - Sonu Kumar
		Nat Cell Biol; Single-cell transcriptomics uncovers endothelial progenitor-like remodelling driving human coronary atherosclerosis progression.; Authors - Fang Yao
		Nat Commun; Generation of nanobodies from transgenic 'LamaMice' lacking an endogenous immunoglobulin repertoire; Authors - Thomas Eden
		Nat Commun; EGFR core fucosylation, induced by hepatitis C virus, promotes TRIM40-mediated-RIG-I ubiquitination and suppresses interferon-I antiviral defenses; Authors - Qiu Pan
	WB	Mol Cell; OXCT1 functions as a succinyltransferase, contributing to hepatocellular carcinoma via succinylating LACTB; Authors - Wenhao Ma