Recombinant Mouse MASP1 protein (rFc Tag)
Species
Mouse
Purity
>90 %, SDS-PAGE
Tag
rFc Tag
Activity
not tested
Cat no : Eg4420
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Mouse MASP1 protein Arg300-Lys445 (Accession# P98064-1) with a rabbit IgG Fc tag at the C-terminus |
| GeneID | 17174 |
| Accession | P98064-1 |
| PredictedSize | 42.2 kDa |
| SDS-PAGE | 45-50 kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
MASP1, or MBL associated serine protease 1, encodes a serine protease that functions as a component of the lectin pathway of complement activation, which is essential for innate and adaptive immune responses. The lectin pathway becomes activated through MASP-1 autoactivation, and the activated MASP-1 is essential for the activation of MASP-2 and probably MASP-3 (PMID: 27535802). MASP-1 and thrombin effects are synergistic on clot formation (PMID: 26645987).
References:
1. Dobó J. et al. (2016). Sci Rep. 6:31877. 2. Jenny L. et al. (2015). PLoS One. 10(12):e0144633.