Recombinant Mouse OX40L/TNFSF4 protein (rFc Tag)

Species

Mouse

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg1573

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Validation Data Gallery

• 

  Purity of Recombinant Mouse OX40L was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Mouse OX40L protein Ser51-Leu198 (Accession# P43488) with a rabbit IgG Fc tag at the N-terminus.
• GeneID: 22164
• Accession: P43488
• PredictedSize: 43.7 kDa
• SDS-PAGE: 45-55 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

The OX40 ligand (OX40L) is also named as TNFSF4, CD252, Glycoprotein Gp34 and TXGP1. It belongs to the tumor necrosis factor family. OX40L is a costimulatory molecules that is mainly expressed on APCs, including mature DCs, B cells, and macrophages. OX40L interacts with OX40 (also CD134) that is preferentially expressed on activated CD4 T cells. OX40L expression in DCs is up-regulated by several stimulatory signalings, including thymic stromal lymphopoietin (TSLP), CD40, PAMPs, and IFN-α. Of note, IgE-Ag complex-activated mast cells up-regulate the expression of OX40L, and interactions between OX40L on mast cells and OX40 on T cells is required for T cell proliferation.

References:

1.Sugamura K. et al. (2004) Nat Rev Immunol. 4(6):420-31.
2.Yashiro T. et al. (2016) Sci Rep. 6:34825.
3.Kurche JS. et al. (2012) J Immunol. 188(2):585-93. 
4.Ito T. et al. (2005) J Exp Med. 202(9):1213-23.
5.Nakae S. et al. (2006) J Immunol. 176(4):2238-48.