Recombinant Mouse SIGNR1/CD209b protein (His Tag)

Species

Mouse

Purity

>90 %, SDS-PAGE

Tag

His Tag

Activity

not tested

Cat no : Eg1497

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Validation Data Gallery

• 

  Purity of Recombinant Mouse SIGNR1 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Mouse SIGNR1 protein Gln74-Gly325 (Accession# Q8CJ91-1) with a His tag at the N-terminus.
• GeneID: 69165
• Accession: Q8CJ91-1
• PredictedSize: 30.3 kDa
• SDS-PAGE: 30-40 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

SIGNR1 (CD209b) is a mouse homolog of the human DC-SIGN receptor. SIGNR1 is a type II transmembrane protein with an extracellular domain consisting of a neck region made up of four repeats of 28 amino acids and a carbohydrate recognition domain. It is expressed on marginal zone macrophages and peritoneal macrophages and is a key receptor for the recognition and efficient clearance of pathogens by the innate immune system.

References:

1.Park CG, et al. (2001) Int Immunol. 13(10):1283-90.
2.Lanoue A, et al. (2004) J Exp Med. 200(11):1383-93.