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Recombinant Mouse Serpin E1/PAI-1 protein (His Tag)

Species

Mouse

Purity

>90 %, SDS-PAGE

Tag

His Tag

Activity

not tested

Cat no : Eg1100

Validation Data Gallery

  • Purity of Recombinant Mouse Serpin E1 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse Serpin E1 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Mouse Serpin E1 protein Thr23-Pro402 (Accession# P22777) with a His tag at the C-terminus.
GeneID 18787
Accession P22777
PredictedSize 44.0 kDa
SDS-PAGE 42-48 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Serpin E1, also known as plasminogen activator inhibitor 1 (PAI-1), is a protein that belongs to the serpin family of serine protease inhibitors. It is primarily produced by endothelial cells and plays a crucial role in the regulation of fibrinolysis by inhibiting the activity of plasminogen activators, such as tissue plasminogen activator (tPA) and urokinase (uPA). Serpin E1 is involved in a variety of physiological and pathological processes, including fibrinolysis, fibrosis, angiogenesis, wound healing, and the invasion and metastasis of tumor cells.

References:

1. Catarinella G. et al. (2022) Cell Death Dis. 13(8):737.
2. Meltzer ME. et al. (2010) Blood. 116(1):113-21.
3. Janciauskiene S. et al. (2024) Biomed Pharmacother. 175:116618.