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MultiProTM 5CFLX Anti-Human CD31 (3F8E2)

CD31 Monoclonal Antibody for

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

Conjugate

5CFLX

CloneNo.

3F8E2

Cat no : G66065-2-5C

Synonyms

CD31, EndoCAM, FLJ58394, GPIIA, PECA1, PECAM 1, PECAM1, VEC marker

Validation Data Gallery

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  • Single Cell Sequencing experiment G66065-2-5C on Resting PBMC

    Single Cell Sequencing experiment G66065-2-5C on Resting PBMC

    G66065-2-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G66065-2-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G66065-2-5C on PBMC treated with 4hr LPS + TI

    G66065-2-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G66065-2-5C on Resting PBMC

    Single Cell Sequencing experiment G66065-2-5C on Resting PBMC

    G66065-2-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Stain-Fix protocol.

Tested Applications

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product.
Positive Single Cell detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product.

Recommended dilution

ApplicationDilution
SINGLE CELL (INTRA)<0.5ug/test
SINGLE CELL<0.5ug/test
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

G66065-2-5C targets CD31 in applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Oligo Conjugate
Type Monoclonal
Immunogen CD31 fusion protein Ag19730
Full Name MultiProTM 5CFLX Anti-Human CD31 (3F8E2)
Calculated Molecular Weight 83 kDa
GenBank Accession NumberBC022512
Gene Symbol PECAM1
Gene ID (NCBI) 5175
Conjugate 5CFLX
Form Liquid
Storage Buffer PBS with 1mM EDTA and 0.09% sodium azide
Storage Conditions2-8°C

Background Information

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).

Protocols

MultiPro™ Cell Surface and Intracellular Staining ProtocolDownload protocol
10x Genomics Cell Surface Protein Only Staining ProtocolDownload protocol