MultiProTM 5CFLX Anti-Human Phospho-Beta Catenin (Ser675) (6D6)

Phospho-Beta Catenin (Ser675) Recombinant Antibody for Single Cell (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

Single Cell (Intra)

Conjugate

5CFLX

CloneNo.

6D6

Cat no : G80084-1-5C

Synonyms

b cat, Beta catenin, Catenin beta 1, CTNNB, CTNNB1, DKFZp686D02253, FLJ25606, FLJ37923, PRO2286



Tested Applications

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product.

Recommended dilution

ApplicationDilution
SINGLE CELL (INTRA)<0.5ug/test
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

G80084-1-5C targets Phospho-Beta Catenin (Ser675) in Single Cell (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Oligo Conjugate
Type Recombinant
Immunogen Peptide
Full Name MultiProTM 5CFLX Anti-Human Phospho-Beta Catenin (Ser675) (6D6)
Calculated Molecular Weight 781 aa, 86 kDa
GenBank Accession NumberBC058926
Gene Symbol Beta Catenin
Gene ID (NCBI) 1499
Conjugate 5CFLX
Form Liquid
Storage Buffer PBS with 1mM EDTA and 0.09% sodium azide
Storage Conditions2-8°C

Background Information

β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin was originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222). PKA was shown to phosphorylate β-catenin at Ser675. Phosphorylation at Ser675 induces β-catenin accumulation in the nucleus and increases its transcriptional activity.

Protocols

MultiPro™ Cell Surface and Intracellular Staining ProtocolDownload protocol
10x Genomics Cell Surface Protein Only Staining ProtocolDownload protocol