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N-cadherin Polyclonal antibody
N-cadherin Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (3)
Applications
WB, IP, IHC, IF, FC,ELISA
Conjugate
Unconjugated
334
Cat no : 22018-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | rat brain tissue, C2C12 cells, HEK-293 cells, mouse brain tissue, mouse heart tissue, C6 cells, rat heart tissue, HeLa cells |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse heart tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | mouse heart tissue, C2C12 cells |
| Positive FC detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:3000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 308 publications below |
| IHC | See 32 publications below |
| IF | See 49 publications below |
| FC | See 1 publications below |
Product Information
The immunogen of 22018-1-AP is N-cadherin Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | bovine, gecko, horse, human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | N-cadherin fusion protein Ag16792 |
| Full Name | cadherin 2, type 1, N-cadherin (neuronal) |
| Calculated molecular weight | 906 aa, 100 kDa |
| Observed molecular weight | 130 kDa |
| GenBank accession number | BC036470 |
| Gene symbol | CDH2 |
| Gene ID (NCBI) | 1000 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Neuronal cadherin (N-cadherin), also known as cadherin-2 (CDH2), is a calcium-binding protein that mediates cell-cell adhesions of neuronal and some non-neuronal cell types.
What is the molecular weight of N-cadherin? Is N-cadherin post-translationally modified?
The molecular weight of mature N-cadherin is 127 kDa. N-cadherin is synthesized in a precursor form that undergoes proteolytic cleavage by furin at the Golgi apparatus. Additionally, it can be phosphorylated by casein kinase II and N-glycosylated, which affects its stability (PMID: 12604612 and 19846557).
What is the subcellular localization of N-cadherin? What is the tissue expression pattern of N-cadherin?
N-cadherin is an integral membrane protein present at the plasma membrane, forming adherens junctions. It is widely expressed in the nervous system, where it flanks the active zone of synapses and is important for synapse formation and remodeling. It is also present in the lens, skeletal, and cardiac muscles (PMID: 3857614). In the muscle, N-cadherin plays a role in myoblast differentiation, while in the heart it is required for the formation of intercalated discs. Additionally, N-cadherin is present in blood vessels, promoting angiogenesis by forming adhesive complexes between endothelial cells and pericytes (PMID: 24521477).
What is the role of N-cadherin during the epithelial-mesenchymal transition (EMT)?
EMT is a crucial process during gastrulation that leads to the formation of mesenchymal cells. It is marked by decreased expression of E-cadherin and upregulation of N-cadherin, which promotes cell migration (PMID: 23481201). Similarly, upregulation of N-cadherin is observed in many cancer cell types and is associated with increased invasiveness and metastasis.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for N-cadherin antibody 22018-1-AP | Download protocol |
| IHC protocol for N-cadherin antibody 22018-1-AP | Download protocol |
| IF protocol for N-cadherin antibody 22018-1-AP | Download protocol |
| IP protocol for N-cadherin antibody 22018-1-AP | Download protocol |
| FC protocol for N-cadherin antibody 22018-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Dose Response Low-Dose Radiation Enhanced Inhibition of Breast Tumor Xenograft and Reduced Myocardial Injury Induced by Doxorubicin. | ||
J Exp Clin Cancer Res Tobacco smoke induced hepatic cancer stem cell-like properties through IL-33/p38 pathway. | ||
Mol Ther Nucleic Acids The Attenuation of Trophoblast Invasion Caused by the Downregulation of EZH2 Is Involved in the Pathogenesis of Human Recurrent Miscarriage. | ||
Eur Rev Med Pharmacol Sci Opa interacting protein 5 promotes metastasis of nasopharyngeal carcinoma cells by promoting EMT via modulation of JAK2/STAT3 signal. | ||
Urol Oncol RPS7 promotes cell migration through targeting epithelial-mesenchymal transition in prostate cancer. | ||
Hypertens Res The potential role of pregnancy-associated plasma protein-A2 in angiogenesis and development of preeclampsia. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Lianjie (Verified Customer) (07-26-2019) | Works very well.
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FH Aurelie (Verified Customer) (06-13-2019) | Great antibody, no background, works also in human U2OS and primary fibroblasts with the same efficiency.
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