Tested Applications
| Positive WB detected in | mouse brain tissue, A549 cells, C2C12 cells, HEK-293 cells, rat brain tissue, HepG2 cells, NCI-H1299 cells, HeLa cells, C6 cells, PC-3 cells, rat heart tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse heart tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse heart tissue, C2C12 cells |
| Positive IF-Fro detected in | mouse heart tissue |
| Positive IF/ICC detected in | C2C12 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)-FRO | IF-FRO : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 1261 publications below |
| IHC | See 167 publications below |
| IF | See 141 publications below |
| FC | See 3 publications below |
| CoIP | See 2 publications below |
Product Information
22018-1-AP targets N-cadherin in WB, IHC, IF/ICC, IF-P, IF-Fro, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, canine, bovine, hamster, horse, gecko |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | N-cadherin fusion protein Ag16792 Predict reactive species |
| Full Name | cadherin 2, type 1, N-cadherin (neuronal) |
| Calculated Molecular Weight | 906 aa, 100 kDa |
| Observed Molecular Weight | 130 kDa |
| GenBank Accession Number | BC036470 |
| Gene Symbol | N-cadherin |
| Gene ID (NCBI) | 1000 |
| RRID | AB_2813891 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P19022 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Neuronal cadherin (N-cadherin), also known as cadherin-2 (CDH2), is a calcium-binding protein that mediates cell-cell adhesions of neuronal and some non-neuronal cell types.
What is the molecular weight of N-cadherin? Is N-cadherin post-translationally modified?
The molecular weight of mature N-cadherin is 127 kDa. N-cadherin is synthesized in a precursor form that undergoes proteolytic cleavage by furin at the Golgi apparatus. Additionally, it can be phosphorylated by casein kinase II and N-glycosylated, which affects its stability (PMID: 12604612 and 19846557).
What is the subcellular localization of N-cadherin? What is the tissue expression pattern of N-cadherin?
N-cadherin is an integral membrane protein present at the plasma membrane, forming adherens junctions. It is widely expressed in the nervous system, where it flanks the active zone of synapses and is important for synapse formation and remodeling. It is also present in the lens, skeletal, and cardiac muscles (PMID: 3857614). In the muscle, N-cadherin plays a role in myoblast differentiation, while in the heart it is required for the formation of intercalated discs. Additionally, N-cadherin is present in blood vessels, promoting angiogenesis by forming adhesive complexes between endothelial cells and pericytes (PMID: 24521477).
What is the role of N-cadherin during the epithelial-mesenchymal transition (EMT)?
EMT is a crucial process during gastrulation that leads to the formation of mesenchymal cells. It is marked by decreased expression of E-cadherin and upregulation of N-cadherin, which promotes cell migration (PMID: 23481201). Similarly, upregulation of N-cadherin is observed in many cancer cell types and is associated with increased invasiveness and metastasis.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for N-cadherin antibody 22018-1-AP | Download protocol |
| IHC protocol for N-cadherin antibody 22018-1-AP | Download protocol |
| IF protocol for N-cadherin antibody 22018-1-AP | Download protocol |
| IP protocol for N-cadherin antibody 22018-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cancer lncRNA ZNRD1-AS1 promotes malignant lung cell proliferation, migration, and angiogenesis via the miR-942/TNS1 axis and is positively regulated by the m6A reader YTHDC2 | ||
ACS Nano Cancer-Erythrocyte Hybrid Membrane-Camouflaged Magnetic Nanoparticles with Enhanced Photothermal-Immunotherapy for Ovarian Cancer. | ||
ACS Nano Biomimetic Nanomedicine Targeting Orchestrated Metabolism Coupled with Regulatory Factors to Disrupt the Metabolic Plasticity of Breast Cancer | ||
Nat Commun Parvimonas micra promotes oral squamous cell carcinoma metastasis through TmpC-CKAP4 axis | ||
Nat Commun Schwann cells regulate tumor cells and cancer-associated fibroblasts in the pancreatic ductal adenocarcinoma microenvironment | ||
Mol Cancer CircGPR137B/miR-4739/FTO feedback loop suppresses tumorigenesis and metastasis of hepatocellular carcinoma. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH CHI (Verified Customer) (11-25-2024) | The antibody works great for IF
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FH Greta (Verified Customer) (02-08-2024) | Good antibody for WB
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FH Sarah (Verified Customer) (01-04-2024) | Signal at 1:500 was very weak and grainy by immunofluorescence of fixed HCT116 cells.
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FH Sarah (Verified Customer) (02-09-2023) | Worked well for western blot of mouse brain for 1hr at room temp
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FH Ralph (Verified Customer) (05-17-2022) | The antibody works well in indirect immunofluorescence, stains the cell membrane.
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FH Saba (Verified Customer) (02-21-2022) | The band intensity of the antibody is so sharp even in very diluted concentration.
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FH Lianjie (Verified Customer) (07-26-2019) | Works very well.
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FH Aurelie (Verified Customer) (06-13-2019) | Great antibody, no background, works also in human U2OS and primary fibroblasts with the same efficiency.
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