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CoraLite®594-conjugated N-cadherin Polyclonal antibody

N-cadherin Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-22018

Synonyms

Cadherin 2, CD325, CDH2, CDHN, CDw325, N cadherin, NCAD, Neural cadherin



Tested Applications

Positive IF detected inmouse heart tissue
Planning an IHC experiment? We recommend our IHCeasy N-cadherin Ready-To-Use IHC Kit. N-cadherin primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 22018-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of CL594-22018 is N-cadherin Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen N-cadherin fusion protein Ag16792
Full Name cadherin 2, type 1, N-cadherin (neuronal)
Calculated molecular weight 906 aa, 100 kDa
Observed molecular weight 130 kDa
GenBank accession numberBC036470
Gene symbol CDH2
Gene ID (NCBI) 1000
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths593 nm/614 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

Neuronal cadherin (N-cadherin), also known as cadherin-2 (CDH2), is a calcium-binding protein that mediates cell-cell adhesions of neuronal and some non-neuronal cell types. 

What is the molecular weight of N-cadherin? Is N-cadherin post-translationally modified?

The molecular weight of mature N-cadherin is 127 kDa. N-cadherin is synthesized in a precursor form that undergoes proteolytic cleavage by furin at the Golgi apparatus. Additionally, it can be phosphorylated by casein kinase II and N-glycosylated, which affects its stability (PMID: 12604612 and 19846557). 

What is the subcellular localization of N-cadherin? What is the tissue expression pattern of N-cadherin?

N-cadherin is an integral membrane protein present at the plasma membrane, forming adherens junctions. It is widely expressed in the nervous system, where it flanks the active zone of synapses and is important for synapse formation and remodeling. It is also present in the lens, skeletal, and cardiac muscles (PMID: 3857614). In the muscle, N-cadherin plays a role in myoblast differentiation, while in the heart it is required for the formation of intercalated discs. Additionally, N-cadherin is present in blood vessels, promoting angiogenesis by forming adhesive complexes between endothelial cells and pericytes (PMID: 24521477). 

What is the role of N-cadherin during the epithelial-mesenchymal transition (EMT)?

EMT is a crucial process during gastrulation that leads to the formation of mesenchymal cells. It is marked by decreased expression of E-cadherin and upregulation of N-cadherin, which promotes cell migration (PMID: 23481201). Similarly, upregulation of N-cadherin is observed in many cancer cell types and is associated with increased invasiveness and metastasis.

Protocols

Product Specific Protocols
IF protocol for CL594 N-cadherin antibody CL594-22018Download protocol
Standard Protocols
Click here to view our Standard Protocols