Filter: AllWBIHCIF/ICC

WB analysis of NIH/3T3 using 15548-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15548-1-AP (NADK Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of RAW264.7 using 15548-1-AP
RAW264.7 cells were subjected to SDS PAGE followed by western blot with 15548-1-AP (NADK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 15548-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15548-1-AP (NADK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 15548-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 15548-1-AP (NADK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 15548-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 15548-1-AP (NADK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 15548-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15548-1-AP (NADK Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of human kidney using 15548-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15548-1-AP (NADK antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human kidney using 15548-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15548-1-AP (NADK antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human testis using 15548-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 15548-1-AP (NADK antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human testis using 15548-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 15548-1-AP (NADK antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human brain using 15548-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 15548-1-AP (NADK antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human brain using 15548-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 15548-1-AP (NADK antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human lung using 15548-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 15548-1-AP (NADK antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human lung using 15548-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 15548-1-AP (NADK antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human ovary using 15548-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 15548-1-AP (NADK antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human ovary using 15548-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 15548-1-AP (NADK antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HeLa using 15548-1-AP
Immunofluorescent analysis of Hela cells, using NADK antibody 15548-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Featured Product
KD/KO Validated

NADK Polyclonal antibody

Name: NADK Polyclonal antibody
Brand: Proteintech
SKU: 15548-1-AP

NADK Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 15548-1-AP

Publications(12)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Synonyms

Poly(P)/ATP NAD kinase, NAD kinase, EC:2.7.1.23

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Overview

Tested Applications
Product Information
Publications (12)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	NIH/3T3 cells, HeLa cells, RAW264.7
Positive IHC detected in	human kidney tissue, human testis tissue, human brain tissue, human lung tissue, human ovary tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	Hela cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 7 publications below
IHC	See 5 publications below
IF	See 3 publications below
CoIP	See 1 publications below

Product Information

15548-1-AP targets NADK in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag7906 Product name: Recombinant human NADK protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 98-446 aa of BC001709 Sequence: LTWNKSPKSVLVIKKMRDASLLQPFKELCTHLMEENMIVYVEKKVLEDPAIASDESFGAVKKKFCTFREDYDDISNQIDFIICLGGDGTLLYASSLFQGSVPPVMAFHLGSLGFLTPFSFENFQSQVTQVIEGNAAVVLRSRLKVRVVKELRGKKTAVHNGLGEKGSQAAGLDMDVGKQAMQYQVLNEVVIDRGPSSYLSNVDVYLDGHLITTVQGDGVIVSTPTGSTAYAAAAGASMIHPNVPAIMITPICPHSLSFRPIVVPAGVELKIMLSPEARNTAWVSFDGRKRQEIRHGDSISITTSCYPLPSICVRDPVSDWFESLAQCLHWNVRKKQAHFEEEEEEEEEG Predict reactive species
Full Name	NAD kinase
Calculated Molecular Weight	49 kDa
Observed Molecular Weight	49 kDa
GenBank Accession Number	BC001709
Gene Symbol	NADK
Gene ID (NCBI)	65220
RRID	AB_2147345
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O95544
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NAD kinase (NADK) is the sole NADP+-biosynthetic enzyme that catalyzes phosphorylation of NAD+ to yield NADP+ using ATP as a phosphoryl donor, and thus, plays a vital role in the cell and represents a potentially powerful antimicrobial drug target (PMID:21526340). It belongs to the NAD kinase family. NADK has 3 isoforms with the molecular mass of 49, 63 and 46 kDa. The catalytically active human NADK is a homotetramer (PMID:11594753).

Protocols

Product Specific Protocols
IF protocol for NADK antibody 15548-1-AP	Download protocol
IHC protocol for NADK antibody 15548-1-AP	Download protocol
WB protocol for NADK antibody 15548-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse,human	IF	Cell Metab Upregulation of Antioxidant Capacity and Nucleotide Precursor Availability Suffices for Oncogenic Transformation. Authors - Yang Zhang KD Validated View Article
human	IHC	Sci Adv G6PD-mediated increase in de novo NADP+ biosynthesis promotes antioxidant defense and tumor metastasis. Authors - Yang Zhang View Article
	WB	Nucleic Acids Res Human DNA ligase IV is able to use NAD+ as an alternative adenylation donor for DNA ends ligation. Authors - Shih-Hsun Chen View Article
human	IHC	Technol Cancer Res Treat A Pyroptosis-Related Gene Signature Predicts Prognosis and Tumor Immune Microenvironment in Colorectal Cancer Authors - Linjing Li View Article
human	WB	ACS Omega Profiling of the Peripheral Blood Mononuclear Cells Proteome by Shotgun Proteomics Identifies Alterations of Immune System Components, Proteolytic Balance, Autophagy, and Mitochondrial Metabolism in Glaucoma Subjects Authors - Sara Giammaria View Article
human	WB,IHC	Clin Exp Hepatol NADK as a molecular marker to distinguish between alcohol- and non-alcohol-associated liver cirrhosis: A pilot study Authors - Ki-Hoon Park View Article