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  • KD/KO Validated

NADK Polyclonal antibody

NADK Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 15548-1-AP


NAD kinase, NADK, Poly(P)/ATP NAD kinase

Tested Applications

Positive WB detected inNIH/3T3 cells, HeLa cells, RAW264.7
Positive IHC detected inhuman kidney tissue, human testis tissue, human brain tissue, human lung tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15548-1-AP targets NADK in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NADK fusion protein Ag7906
Full Name NAD kinase
Calculated Molecular Weight 49 kDa
Observed Molecular Weight 49 kDa
GenBank Accession NumberBC001709
Gene Symbol NADK
Gene ID (NCBI) 65220
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NAD kinase (NADK) is the sole NADP+-biosynthetic enzyme that catalyzes phosphorylation of NAD+ to yield NADP+ using ATP as a phosphoryl donor, and thus, plays a vital role in the cell and represents a potentially powerful antimicrobial drug target (PMID:21526340). It belongs to the NAD kinase family. NADK has 3 isoforms with the molecular mass of 49, 63 and 46 kDa. The catalytically active human NADK is a homotetramer (PMID:11594753).


Product Specific Protocols
WB protocol for NADK antibody 15548-1-APDownload protocol
IHC protocol for NADK antibody 15548-1-APDownload protocol
IF protocol for NADK antibody 15548-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Cell Metab

Upregulation of Antioxidant Capacity and Nucleotide Precursor Availability Suffices for Oncogenic Transformation.

Authors - Yang Zhang
  • KD Validated

Sci Adv

G6PD-mediated increase in de novo NADP+ biosynthesis promotes antioxidant defense and tumor metastasis.

Authors - Yang Zhang

Nucleic Acids Res

Human DNA ligase IV is able to use NAD+ as an alternative adenylation donor for DNA ends ligation.

Authors - Shih-Hsun Chen

Nat Commun

NADP+ is an endogenous PARP inhibitor in DNA damage response and tumor suppression.

Authors - Chunjing Bian

J Biol Chem

Glucose-6-phosphate dehydrogenase exerts anti-stress effects independently of its enzymatic activity

Authors - Xiaohan Jin

Redox Biol

NADK-mediated de novo NADP(H) synthesis is a metabolic adaptation essential for breast cancer metastasis

Authors - Didem Ilter