NADK Polyclonal antibody

• Featured Product
• KD/KO Validated

NADK Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 15548-1-AP

Synonyms

NAD kinase, NADK, Poly(P)/ATP NAD kinase

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Validation Data Gallery

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  WB analysis of NIH/3T3 using 15548-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15548-1-AP (NADK Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 15548-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15548-1-AP (NADK Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15548-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15548-1-AP (NADK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW264.7 using 15548-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 15548-1-AP (NADK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 15548-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15548-1-AP (NADK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15548-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15548-1-AP (NADK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 15548-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15548-1-AP (NADK antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 15548-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15548-1-AP (NADK antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human testis using 15548-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15548-1-AP (NADK antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 15548-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15548-1-AP (NADK antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 15548-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15548-1-AP (NADK antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 15548-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15548-1-AP (NADK antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 15548-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15548-1-AP (NADK antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 15548-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15548-1-AP (NADK antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary using 15548-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15548-1-AP (NADK antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary using 15548-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15548-1-AP (NADK antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 15548-1-AP

  Immunofluorescent analysis of Hela cells, using NADK antibody 15548-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: NIH/3T3 cells, HeLa cells, RAW264.7
• Positive IHC detected in: human kidney tissue, human testis tissue, human brain tissue, human lung tissue, human ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below

Product Information

15548-1-AP targets NADK in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NADK fusion protein Ag7906
• Full Name: NAD kinase
• Calculated Molecular Weight: 49 kDa
• Observed Molecular Weight: 49 kDa
• GenBank Accession Number: BC001709
• Gene Symbol: NADK
• Gene ID (NCBI): 65220
• RRID: AB_2147345
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NAD kinase (NADK) is the sole NADP+-biosynthetic enzyme that catalyzes phosphorylation of NAD+ to yield NADP+ using ATP as a phosphoryl donor, and thus, plays a vital role in the cell and represents a potentially powerful antimicrobial drug target (PMID:21526340). It belongs to the NAD kinase family. NADK has 3 isoforms with the molecular mass of 49, 63 and 46 kDa. The catalytically active human NADK is a homotetramer (PMID:11594753).

Publications

Species	Application	Title
mouse,human	IF	Cell Metab; Upregulation of Antioxidant Capacity and Nucleotide Precursor Availability Suffices for Oncogenic Transformation.; Authors - Yang Zhang ; KD Validated
human	IHC	Sci Adv; G6PD-mediated increase in de novo NADP+ biosynthesis promotes antioxidant defense and tumor metastasis.; Authors - Yang Zhang
	WB	Nucleic Acids Res; Human DNA ligase IV is able to use NAD+ as an alternative adenylation donor for DNA ends ligation.; Authors - Shih-Hsun Chen
human	IF	Nat Commun; NADP+ is an endogenous PARP inhibitor in DNA damage response and tumor suppression.; Authors - Chunjing Bian
human	WB	J Biol Chem; Glucose-6-phosphate dehydrogenase exerts anti-stress effects independently of its enzymatic activity; Authors - Xiaohan Jin
human	IF	Redox Biol; NADK-mediated de novo NADP(H) synthesis is a metabolic adaptation essential for breast cancer metastasis; Authors - Didem Ilter