NCKIPSD Polyclonal antibody

• Featured Product
• KD/KO Validated

NCKIPSD Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 11367-1-AP

Synonyms

AF3P21, Dia interacting protein 1, DIP, DIP 1, DIP1, NCKIPSD, ORF1, SH3 adapter protein SPIN90, SPIN90, VIP54, WASLBP, WISH

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Validation Data Gallery

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  WB analysis of mouse brain using 11367-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11367-1-AP (NCKIPSD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 11367-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11367-1-AP (NCKIPSD antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of fetal human brain using 11367-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 11367-1-AP (NCKIPSD Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 11367-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11367-1-AP (NCKIPSD Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 11367-1-AP

  IP result of anti-NCKIPSD (IP:11367-1-AP, 4ug; Detection:11367-1-AP 1:300) with mouse brain tissue lysate 4000ug.

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  IHC staining of human breast cancer using 11367-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11367-1-AP (NCKIPSD Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11367-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11367-1-AP (NCKIPSD Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, fetal human brain tissue, human heart tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

11367-1-AP targets NCKIPSD in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NCKIPSD fusion protein Ag1915
• Full Name: NCK interacting protein with SH3 domain
• Calculated Molecular Weight: 715 aa, 78 kDa
• Observed Molecular Weight: 80 kDa
• GenBank Accession Number: BC016052
• Gene Symbol: NCKIPSD
• Gene ID (NCBI): 51517
• RRID: AB_2150732
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human,mouse	WB,IHC	Cancer Res; SPIN90 depletion and Microtubule Acetylation Mediate Stromal Fibroblast Activation in Breast Cancer Progression.; Authors - Eunae You ; KO Validated