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  • KD/KO Validated

SRC3/NCOA3 Polyclonal antibody

SRC3/NCOA3 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 20032-1-AP

Synonyms

ACTR, AIB 1, AIB1, CBP interacting protein, KAT13B, NCoA 3, NCOA3, nuclear receptor coactivator 3, pCIP, SRC 3, SRC3, SRC3/NCOA3, TRAM 1, TRAM1



Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

20032-1-AP targets SRC3/NCOA3 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name nuclear receptor coactivator 3
Calculated molecular weight 155 kDa
Observed molecular weight 160 kDa
GenBank accession numberNM_006534
Gene symbol NCOA3
Gene ID (NCBI) 8202
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NCOA3, also named as AIB1, SRC3 and TRAM1, belongs to the SRC/p160 nuclear receptor coactivator family. NCOA3 is a nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. NOCA3 plays a central role in creating a multisubunit coactivator complex, which probably acts via remodeling of chromatin. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ER), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). NCOA3 also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit. The antibody can recognizes all the isoforms of NCOA3.

Protocols

Product Specific Protocols
WB protocol for SRC3/NCOA3 antibody 20032-1-APDownload protocol
IHC protocol for SRC3/NCOA3 antibody 20032-1-APDownload protocol
IF protocol for SRC3/NCOA3 antibody 20032-1-APDownload protocol
FC protocol for SRC3/NCOA3 antibody 20032-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Biomed Pharmacother

PMID: 32036219

Authors - Yu Chen
  • KD Validated
humanWB

J Biol Chem

A potential target for liver cancer management, lysophosphatidic acid receptor 6 (LPAR6), is transcriptionally up-regulated by the NCOA3 coactivator.

Authors - Xuan Zheng
  • KD Validated